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Katalogové číslo: (USBI040210-HRP)
Dodavatel: US Biological
Popis: Anti-PNKP Rabbit Polyclonal Antibody (HRP (Horseradish Peroxidase))
Měrná jednotka: 1 * 200 µl


Katalogové číslo: (0307.1000)
Dodavatel: Avantor
Popis: Fosforečnan sodný dodekahydrát, BAKER ANALYZED® ACS, J.T.Baker®
Měrná jednotka: 1 * 1 kg

Dodavatel: VWR Chemicals
Popis: Fosforečnan sodný dodekahydrát 98.0-102.0%, AnalaR NORMAPUR® Reag. Ph.Eur., ACS
Katalogové číslo: (ABCAAB124980-1ML)
Dodavatel: Abcam
Popis: Anti-NUP98 Rabbit Monoclonal Antibody [clone: EPR6678]
Měrná jednotka: 1 * 1 mL

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Katalogové číslo: (BOSSBS-10235R-HRP)
Dodavatel: Bioss
Popis: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
Měrná jednotka: 1 * 100 µl


Katalogové číslo: (ACRO446222500)
Dodavatel: Thermo Fisher Scientific
Popis: Dihydrogenfosforečnan sodný dihydrát for HPLC
Měrná jednotka: 1 * 250 g

Dodavatel: Avantor
Popis: Fosforečnan sodný dodekahydrát 98.0-102.0%, crystals, AR® ACS, Macron Fine Chemicals™

Katalogové číslo: (BOSSBS-10235R-A488)
Dodavatel: Bioss
Popis: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
Měrná jednotka: 1 * 100 µl


Dodavatel: Thermo Fisher Scientific
Popis: Fosforečnan sodný dodekahydrát ≥97%
Katalogové číslo: (BOSSBS-10235R-A750)
Dodavatel: Bioss
Popis: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumourigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
Měrná jednotka: 1 * 100 µl


Katalogové číslo: (BOSSBS-10235R-CY5)
Dodavatel: Bioss
Popis: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
Měrná jednotka: 1 * 100 µl


Katalogové číslo: (MEMMUN30PAH8)
Dodavatel: MEMMERT
Popis: Temperature sensors for Memmert UNpa ovens
Měrná jednotka: 1 * 1 KS


Dodavatel: Thermo Fisher Scientific
Popis: Dihydrogenfosforečnan sodný dihydrát 98%, Extra Pure
Dodavatel: Thermo Fisher Scientific
Popis: Fosforečnan sodný dodekahydrát, Extra Pure
Dodavatel: Thermo Fisher Scientific
Popis: Dihydrogenfosforečnan sodný monohydrát 98.0-102.0% ACS
Dodavatel: Thermo Fisher Scientific
Popis: Dihydrogenfosforečnan sodný monohydrát ≥99.998% (metals basis), Puratronic®
Cena na vyžádání
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Daná chemikálie je regulována dle platné legislativy a bude vyžadováno vyplnění formuláře. Jeho včasným vyplněním uspíšíte dodání produktu.
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
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Původní produkt již není k dispozici. Zobrazen je náhradní produkt.
Tento produkt již není k dispozici. Podobné produkty můžete vyhledat pomocí VWR katalogových čísel nebo čísel dodavatele uvedených výše. Pokud potřebujete další asistenci, zavolejte na telefonní číslo zákaznického servisu VWR 321 570 321.
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