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Methylene blue trihydrate, powder C.I. 52015

Katalogové číslo: (ICNA04800663)

Dodavatel: MP Biomedicals

Popis: Methylene blue has been used as a stain in bacteriology and as an oxidation-reduction indicator. A sensitive stain for RNA and
ribonuclease. An ethidium bromide alternative for the visualization of nucleic acids in gels:

Měrná jednotka: 1 * 25 g

Bezpečnostní list Certifikáty Akce

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Vizmut ≥99.9999% (metals basis), rods, Puratronic®, Length: 145 mm (5.71 in), Ø 10 mm (0.39 in)

Katalogové číslo: (47229.KF)

Dodavatel: Thermo Fisher Scientific

Popis: Vizmut ≥99.9999% (metals basis), rods, Puratronic®, Length: 145 mm (5.71 in), Ø 10 mm (0.39 in)

Měrná jednotka: 1 * 1 KS

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Nitro modř tetrazolium for molecular biology

Dodavatel: MP Biomedicals

Popis: p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.

p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.

2,3,5-Trifenyltetrazoliumchlorid, white powder

Dodavatel: MP Biomedicals

Popis: 2,3,5-Triphenyltetrazolium Chloride is a redox indicator. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains as white TTC in areas of necrosis since these enzymes have been either denatured or degraded.
2,3,5-Triphenyltetrazolium Chloride is commonly used in biochemical experiments especially to indicate cellular respiration. TTC has been employed in autopsy pathology to assist identification of post-mortem myocardial infarctions. Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase; while areas of potential infarctions will be more pale. Useful indicator for reducing substances