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Product Image-K1232

PCR cloning kit, CloneJET

Dodavatel: Thermo Fisher Scientific
Popis: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Product Image-EL0016

T4 DNA ligase

Katalogové číslo: (EL0016)
Dodavatel: Thermo Fisher Scientific
Popis: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
Měrná jednotka: 1 * 1 KIT

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Nové řešení transparentnosti pro evropské zákazníky

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Image Unavailable-70573-3

Ligation premix, Clonables™

Katalogové číslo: (70573-3)
Dodavatel: Merck Millipore (Novagen)
Popis: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.
Měrná jednotka: 1 * 55 µl
Product Image-K1261

LIC cloning and expression kits, aLICator™, Fermentas

Dodavatel: Thermo Fisher Scientific
Popis: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Product Image-GA1100-10

DNA cloning kits, Gibson Assembly® HiFi 1 step kit

Dodavatel: SGI - DNA Synthetic Genomics
Popis: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

Bezpečnostní list

Product Image-SYGEGA1100-4XS

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Dodavatel: SGI - DNA Synthetic Genomics
Popis: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Product Image-GA1200-50

Gibson Assembly® Ultra kit

Dodavatel: SGI - DNA Synthetic Genomics
Popis: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

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Product Image-27-0361-01

T4 DNA ligase

Katalogové číslo: (27-0361-01)
Dodavatel: Cytiva
Popis: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
Měrná jednotka: 1 * 50 Tests
Product Image-GA1100-10MM

Gibson Assembly® HiFi 1 Step, Master mixes

Dodavatel: SGI - DNA Synthetic Genomics
Popis: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

Bezpečnostní list

Product Image-GA1200-10MM

Gibson Assembly® Ultra, Master mixes

Dodavatel: SGI - DNA Synthetic Genomics
Popis: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

Bezpečnostní list

Product Image-OXNTSQK-LSK114-XL

Ligation Sequencing Kits

Dodavatel: Oxford Nanopore Technologies
Popis: Ligation Sequencing Kits enable ligation-based, PCR-free nanopore sequencing of native DNA.

Certifikáty

Product Image-EDVO300

Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Katalogové číslo: (EDVO300)
Dodavatel: EDVOTEK
Popis: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.
Měrná jednotka: 1 * 1 KIT
Image Unavailable-ENZOALX8049120100

Anti-TRAIL-R1 Mouse Monoclonal Antibody [clone: DJR1]

Katalogové číslo: (ENZOALX8049120100)
Dodavatel: ENZO LIFE SCIENCES
Popis: DR4 is 56kDa member 10A of the TNFR superfamily (TNFRSF10A), also known as TRAIL-R1, Apo-2, and CD261. It is expressed at low levels by activated T cells and some tumors. After TRAIL engagement, DR4 (TRAIL-R1), through activation of NF-κB, induces apoptosis in the TRAIL ligated cell.
Měrná jednotka: 1 * 100 µG

New Product


Image Unavailable-BTIUBNUM0657-50

Anti-KDR Mouse Monoclonal Antibody (Purified, BSA-free) [clone: KDR657]

Katalogové číslo: (BTIUBNUM0657-50)
Dodavatel: Biotium
Popis: CD309, also known as VEGF-R2, KDR3, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, VEGF-D, and VEGF splice isoforms. Ligation of VEGF-R2 with its ligands results in the receptor dimerization and auto-phosphorylation, stimulating endothelial cell proliferation and migration.
Měrná jednotka: 1 * 50 µl
Image Unavailable-ENZOALX8049080100

Anti-TRAIL-R3 Mouse Monoclonal Antibody [clone: DJR3]

Katalogové číslo: (ENZOALX8049080100)
Dodavatel: ENZO LIFE SCIENCES
Popis: DcR1 is a 65kDa GPI-anchored member of the TNFR superfamily (TNFRSF10C) also known as TRAIL-R3, TRID, CD263, and LIT. Although not expressed by most tumors, DcR1 is expressed at low levels on normal tissues, acting as a TRAIL decoy receptor, thus inhibiting apoptosis. DcR1 is termed a decoy receptor, as it does not have a functional death domain to induce apoptosis following TRAIL ligation.
Měrná jednotka: 1 * 100 µG

New Product


Image Unavailable-BNC040721-100

Anti-KDR Rat Monoclonal Antibody (CF405S) [clone: KDR721]

Dodavatel: Biotium
Popis: CD309, also known as VEGFR2, KDR3, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGFR2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGFR2 include VEGF-A, VEGF-C, VEGF-D, and VEGF splice isoforms. Ligation of VEGFR2 with its ligands results in the receptor dimerization and auto-phosphorylation, stimulating endothelial cell proliferation and migration.

Cena na vyžádání
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
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-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
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Tento produkt již není k dispozici. Podobné produkty můžete vyhledat pomocí VWR katalogových čísel nebo čísel dodavatele uvedených výše. Pokud potřebujete další asistenci, zavolejte na telefonní číslo zákaznického servisu VWR 321 570 321.
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