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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-1B1301-KIT

VWR® Protein Precipitation Kit

Katalogové číslo: (1B1301-KIT)
Dodavatel: VWR Chemicals
Popis: The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.
Měrná jednotka: 1 * 1 KIT
Product Image-ROCKMB-061-0100

Conjugate stabilisers

Dodavatel: Rockland Immunochemicals
Popis: Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.

Image Unavailable-ENZOENZ51029K100

eFluxx-ID® multidrug resistance assay kit

Dodavatel: ENZO LIFE SCIENCES
Popis: The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.

Image Unavailable-CAYM10005020-96

Protein carbonyl colorimetric assay kit

Katalogové číslo: (CAYM10005020-96)
Dodavatel: Cayman Chemical
Popis: The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.
Měrná jednotka: 1 * 96 Tests
Product Image-786-892

Bicinchoninic acid (BCA) reducing agent compatible protein Assay

Dodavatel: G-Biosciences
Popis: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.

Certifikáty

Image Unavailable-TNB-1213-L150

Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences

Katalogové číslo: (TNB-1213-L150)
Dodavatel: Tonbo Biosciences
Popis: Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry
Měrná jednotka: 1 * 150 mL
Product Image-786-603

Protein assay kits, RED 660™

Dodavatel: G-Biosciences
Popis: RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

Image Unavailable-33022-1

Thermal shift protein stability kits, GloMelt™

Dodavatel: Biotium
Popis: Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.

Bezpečnostní list

Product Image-786-005

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Katalogové číslo: (786-005)
Dodavatel: G-Biosciences
Popis: A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.
Měrná jednotka: 1 * 1 KIT
Image Unavailable-ENZOBMLUW1000A0001

SUMO-QAPTURE-T® kit for isolation and enrichment of SUMOylated proteins

Katalogové číslo: (ENZOBMLUW1000A0001)
Dodavatel: ENZO LIFE SCIENCES
Popis: The only commercially available kit for isolation and enrichment of SUMOylated proteins.
Měrná jednotka: 1 * 10 Tests
Product Image-ENZOENZ51023KP050

PROTEOSTAT® protein aggregation assay

Katalogové číslo: (ENZOENZ51023KP050)
Dodavatel: ENZO LIFE SCIENCES
Popis: PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.
Měrná jednotka: 1 * 50 Tests
Product Image-CAYM700480-96

Glycogen Assay Kit

Katalogové číslo: (CAYM700480-96)
Dodavatel: Cayman Chemical
Popis: A simple and sensitive tool for assaying glycogen from tissue.
Měrná jednotka: 1 * 1 KS
Product Image-GENO786-890

Bicinchoninic acid (BCA) protein assay

Dodavatel: G-Biosciences
Popis: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

Product Image-786-572

Micro Bicinchoninic Acid (BCA) protein assay

Dodavatel: G-Biosciences
Popis: The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).

Image Unavailable-GENO786-1078

Compatible Lowry protein assay

Dodavatel: G-Biosciences
Popis: These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.

Product Image-CAYM700510-96

L-Lactate Assay Kit

Katalogové číslo: (CAYM700510-96)
Dodavatel: Cayman Chemical
Popis: A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.
Měrná jednotka: 1 * 1 KS
Cena na vyžádání
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Daná chemikálie je regulována dle platné legislativy a bude vyžadováno vyplnění formuláře. Jeho včasným vyplněním uspíšíte dodání produktu.
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
Tento produkt je zablokován vaší organizací. Kontaktujte své nákupní oddělení pro více informací.
Původní produkt již není k dispozici. Zobrazen je náhradní produkt.
Tento produkt již není k dispozici. Podobné produkty můžete vyhledat pomocí VWR katalogových čísel nebo čísel dodavatele uvedených výše. Pokud potřebujete další asistenci, zavolejte na telefonní číslo zákaznického servisu VWR 321 570 321.
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