Hledali jste: Materiál pro výuku biologie

Katalogové číslo: (BE-404)

Dodavatel: G-Biosciences

Popis: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-401)

Dodavatel: G-Biosciences

Popis: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-502)

Dodavatel: G-Biosciences

Popis: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVOS-49)

Dodavatel: EDVOTEK

Popis: Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitochondrial DNA and their biological father from chromosomal DNA.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO282)

Dodavatel: EDVOTEK

Popis: This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO270)

Dodavatel: EDVOTEK

Popis: This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO271)

Dodavatel: EDVOTEK

Popis: An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

Měrná jednotka: 1 * 1 KS

Akce

Dodavatel: EDVOTEK

Popis: Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

Katalogové číslo: (EDVO202)

Dodavatel: EDVOTEK

Popis: Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO212)

Dodavatel: EDVOTEK

Popis: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO124)

Dodavatel: EDVOTEK

Popis: This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO221)

Dodavatel: EDVOTEK

Popis: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO953)

Dodavatel: EDVOTEK

Popis: Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO300)

Dodavatel: EDVOTEK

Popis: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO274)

Dodavatel: EDVOTEK

Popis: Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.

Měrná jednotka: 1 * 1 KS

Bezpečnostní list Akce

Katalogové číslo: (EDVO314)

Dodavatel: EDVOTEK

Popis: Suppressor genes, such as p53, are essential for cell functions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations of genes can either be inherited or accumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation.

Měrná jednotka: 1 * 1 KS

Akce