Hledali jste: MBD

Katalogové číslo: (PRSI29-201)

Dodavatel: ProSci Inc.

Popis: MBD2 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD2 can also repress transcription from methylated gene promoters. MBD2 may function as a mediator of the biological consequences of the methylation signal.It is also reported that the MBD2 functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Měrná jednotka: 1 * 100 µG

Akce

Katalogové číslo: (PRSI55-150)

Dodavatel: ProSci Inc.

Popis: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).

Měrná jednotka: 1 * 400 µl

New Product Akce

Katalogové číslo: (PRSI55-160)

Dodavatel: ProSci Inc.

Popis: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Měrná jednotka: 1 * 400 µl

New Product Akce

Katalogové číslo: (LAUDEMV050)

Dodavatel: LAUDA

Popis: [EN]COMPRESSOR RE004 TYPE: GD 36 MBd 1 * 1 KS

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (ANTIA9015-100)

Dodavatel: ANTIBODIES.COM

Popis: Rabbit polyclonal antibody to MBD3 for WB with samples derived from Human and Mouse.

Měrná jednotka: 1 * 100 µl

New Product Akce

Dodavatel: ANTIBODIES.COM

Popis: Rabbit polyclonal antibody to MeCP2 for WB, ICC/IF and IHC with samples derived from Human, Rat, Mouse and Monkey.

New Product

Katalogové číslo: (ANTIA82510-100)

Dodavatel: ANTIBODIES.COM

Popis: Goat polyclonal antibody to MBD2 for ELISA, WB and IHC with samples derived from human.

Měrná jednotka: 1 * 100 µG

New Product Akce

Katalogové číslo: (ANTIA306017-100)

Dodavatel: ANTIBODIES.COM

Popis: Rabbit monoclonal [ARC2691] antibody to MBD2 for WB with samples derived from Mouse.

Měrná jednotka: 1 * 100 µl

New Product Akce

Katalogové číslo: (ANTIA99392-100)

Dodavatel: ANTIBODIES.COM

Popis: Rabbit polyclonal antibody to MBD3 for WB and ELISA with samples derived from human and mouse.

Měrná jednotka: 1 * 100 µG

New Product Akce

Dodavatel: ANTIBODIES.COM

Popis: Chicken polyclonal antibody to MeCP2 for WB, ICC/IF and IHC with samples derived from human, monkey, rat and mouse.

New Product

Katalogové číslo: (ANTIA249280-100)

Dodavatel: ANTIBODIES.COM

Popis: Mouse monoclonal [CPTC-MBD1-1] antibody to MBD1 for IHC-P with samples derived from human.

Měrná jednotka: 1 * 100 µG

New Product Akce

Katalogové číslo: (ANTIA307276-100)

Dodavatel: ANTIBODIES.COM

Popis: Rabbit monoclonal [ARC2466] antibody to MBD3 for WB and IHC with samples derived from Human, Mouse and Rat.

Měrná jednotka: 1 * 100 µl

New Product Akce

Katalogové číslo: (ANTIA252819-100)

Dodavatel: ANTIBODIES.COM

Popis: Mouse monoclonal [PCRP-MBD3-1C4] antibody to MBD3 for Flow Cytometry and IF with samples derived from Human.

Měrná jednotka: 1 * 100 µG

New Product Akce

Katalogové číslo: (ANTIA249639-100)

Dodavatel: ANTIBODIES.COM

Popis: Mouse monoclonal [PCRP-MBD3-1C4] antibody to MBD3 for flow cytometry and IF with samples derived from human.

Měrná jednotka: 1 * 100 µG

New Product Akce

Dodavatel: ANTIBODIES.COM

Popis: Mouse monoclonal (5A12) antibody to MeCP2 for WB, ICC/IF and IHC with samples derived from human, rat, mouse, canine, bovine, horse and monkey.

New Product

Dodavatel: ANTIBODIES.COM

Popis: Mouse monoclonal (4F11) antibody to MeCP2 for WB, ICC/IF and IHC with samples derived from human, rat, mouse, horse, canine, bovine and porcine.

New Product