Hledali jste: Thermo Fisher Scientific

Katalogové číslo: (R1181)

Dodavatel: Thermo Fisher Scientific

Popis: Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (R0641)

Dodavatel: Thermo Fisher Scientific

Popis: In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA. RNA loading dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis. Formamide also stabilises RNA.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (SM0271)

Dodavatel: Thermo Fisher Scientific

Popis: pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Thermo Fisher Scientific

Popis: T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

Bezpečnostní list

Dodavatel: Thermo Fisher Scientific

Popis: This nuclease-free water is deionized and 0.22 µM membrane-filtered nuclease-free water

Dodavatel: Thermo Fisher Scientific

Popis: Oligo(dT)₁₈ primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Oligo(dT)₁₈ primer has been functionally tested in first strand cDNA synthesis.

Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Katalogové číslo: (EM0821)

Dodavatel: Thermo Fisher Scientific

Popis: CpG methyltransferase enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context. Thermo Scientific M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37 °C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (FD0974)

Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Thermo Fisher Scientific

Popis: ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

Dodavatel: Thermo Fisher Scientific

Popis: O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.

Katalogové číslo: (FD1254)

Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (FD0663)

Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Thermo Fisher Scientific

Popis: FastDigest XbaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest green reaction buffers.

Dodavatel: Thermo Fisher Scientific

Popis: The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Certifikáty

Katalogové číslo: (EP0282)

Dodavatel: Thermo Fisher Scientific

Popis: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.

Měrná jednotka: 1 * 1 KIT

Akce