Hledali jste: Thermo Fisher Scientific

Dodavatel: Thermo Fisher Scientific

Popis: The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Katalogové číslo: (SD0011)

Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific™ Lambda DNA is a temperate<i> E. coli </i> bacteriophage.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (B52)

Dodavatel: Thermo Fisher Scientific

Popis: Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (B49)

Dodavatel: Thermo Fisher Scientific

Popis: Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Měrná jednotka: 1 * 1 KIT

Certifikáty Akce

Dodavatel: Thermo Fisher Scientific

Popis: PCR Master Mix is a 2X concentrated solution of <i>Taq</i> DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

Certifikáty

Dodavatel: Thermo Fisher Scientific

Popis: Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.

Katalogové číslo: (R1021)

Dodavatel: Thermo Fisher Scientific

Popis: Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Thermo Fisher Scientific

Popis: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Katalogové číslo: (FD0644)

Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Thermo Fisher Scientific

Popis: FastDigest™ XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest™ and FastDigest™ Green reaction buffers.

Katalogové číslo: (FD1014)

Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate

Bezpečnostní list

Dodavatel: Thermo Fisher Scientific

Popis: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Dodavatel: Thermo Fisher Scientific

Popis: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Katalogové číslo: (K1441)

Dodavatel: Thermo Fisher Scientific

Popis: 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

Měrná jednotka: 1 * 1 KIT

Akce