Hledali jste: Ed_FR_00007

Katalogové číslo: (BE-414)

Dodavatel: G-Biosciences

Popis: The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO235)

Dodavatel: EDVOTEK

Popis: Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-416)

Dodavatel: G-Biosciences

Popis: The Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic chromatography utilising a hydrophobic enzyme. The use of the enzyme allows purification followed by a simple enzyme assay to detect the fractions that contain the enzyme. This lab activity involves preparation of a crude protein extract and running hydrophobic chromatography to isolate the enzyme.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO957)

Dodavatel: EDVOTEK

Popis: According to the World Health Organisation, between two and three million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-404)

Dodavatel: G-Biosciences

Popis: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO243)

Dodavatel: EDVOTEK

Popis: Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Měrná jednotka: 1 * 1 KS

Bezpečnostní list Akce

Katalogové číslo: (EDVO110)

Dodavatel: EDVOTEK

Popis: This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: EDVOTEK

Popis: Basic concepts of DNA fingerprinting are featured in this Ready-to-Load™ kit by comparing crime scene DNA with suspect DNAs. Fingerprint patterns are separated by agarose gel electrophoresis and the students determine who may have commited the crime.

Katalogové číslo: (EDVO332)

Dodavatel: EDVOTEK

Popis: The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO166)

Dodavatel: EDVOTEK

Popis: An infectious outbreak requires prompt and accurate identification of the biological agent. Often, early clinical symptoms are first identified in exposed individuals and then infectious agents are identified by lab tests. In this experiment, students transmit a simulated infectious agent (chemical dye) between classmates which is only visible under long UV light. The pattern of transmission and primary source is documented.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (763-0001)

Dodavatel: EDVOTEK

Popis: In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO271)

Dodavatel: EDVOTEK

Popis: An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO315)

Dodavatel: EDVOTEK

Popis: Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.

Měrná jednotka: 1 * 1 KS

Bezpečnostní list Akce

Katalogové číslo: (BE-408)

Dodavatel: G-Biosciences

Popis: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of four different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO253)

Dodavatel: EDVOTEK

Popis: In this experiment, students study the diversity of fish using pre-stained, lyophilised proteins. Total protein from perch, walleye and salmon is extracted and pre-stained using an indicator dye. Each fish protein sample has a characteristic banding pattern when separated by denaturing SDS-polyacrylamide gel electrophoresis, which can be used to identify the specific species.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-312)

Dodavatel: G-Biosciences

Popis: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

Měrná jednotka: 1 * 1 KIT

Akce