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Katalogové číslo: (SIAL382485-5G)

Dodavatel: Merck

Popis: 2-Mercapto-5-methoxybenzimidazole, Sigma-Aldrich®

Měrná jednotka: 1 * 5 g

Akce

Dodavatel: Merck

Popis: 2-Hydroxybenzimidazole, Sigma-Aldrich®

Dodavatel: Thermo Fisher Scientific

Popis: X-Gal ≥98%

Bezpečnostní list

Katalogové číslo: (PROOMM0474.03)

Dodavatel: LGC Standards PROMOCHEM

Popis: 2-Hydroxybenzimidazole

Měrná jednotka: 1 * 100 mg

Akce

Dodavatel: MP Biomedicals

Popis: 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate.

Katalogové číslo: (OZBIGX10003)

Dodavatel: OZ BIOSCIENCES

Popis: X-Gal staining kit allows to visualise ß-gal expression through hydrolysis of the X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-Galactopyranoside) substrate yielding blue precipitates.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Thermo Fisher Scientific

Popis: Oxfendazole 98%

New Product

Dodavatel: Thermo Fisher Scientific

Popis: 5,6-Dichlorobenzimidazole-1-β-D-Ribofuranoside 98%

Bezpečnostní list Certifikáty

Katalogové číslo: (OZBIXG31000)

Dodavatel: OZ BIOSCIENCES

Popis: X-Gal substrate is metabolised by the b-galactosidase enzyme. The quality and purity of the substrate is essential to obtain high-quality and reliable results.

Měrná jednotka: 1 * 1 g

Akce

Dodavatel: G-Biosciences

Popis: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Katalogové číslo: (ACRO468041000)

Dodavatel: Thermo Fisher Scientific

Popis: Chemical probes can be used to help establish the
relationship between a molecular target and the broader
biological consequences of modulating that target in cells
or organisms.

Měrná jednotka: 1 * 100 mg

Akce

Katalogové číslo: (J62134.MC)

Dodavatel: Thermo Fisher Scientific

Popis: bisBenzimide H-33342 trihydrochloride trihydrate (Hoechst 33342) 98%

Měrná jednotka: 1 * 100 mg

Bezpečnostní list Certifikáty Akce

Dodavatel: AAT BIOQUEST

Popis: The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy.

Katalogové číslo: (ACRO467991000)

Dodavatel: Thermo Fisher Scientific

Popis: Chemical probes can be used to help establish the
relationship between a molecular target and the broader
biological consequences of modulating that target in cells
or organisms.

Měrná jednotka: 1 * 100 mg

Akce

Katalogové číslo: (AATB17539)

Dodavatel: AAT BIOQUEST

Popis: The long-wavelength tracer nuclear yellow is often combined with the popular retrograde tracer true blue for two-color neuronal mapping.

Měrná jednotka: 1 * 25 mg

Akce

Dodavatel: MP Biomedicals

Popis: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.