Hledali jste: G Bioscienes

Dodavatel: G-Biosciences

Popis: T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. <i>E.coli</i> cells expressing recombinant T4 UvsX gene.

Katalogové číslo: (GENO786-1992)

Dodavatel: G-Biosciences

Popis: T4 recombination mediator protein, UvsY plays important roles in homologous recombination (HR). During HR, the UvsX recombinase competes with the prebound single-stranded binding protein gp32 for DNA-binding sites and UvsY stimulates this filament nucleation event.

Měrná jednotka: 1 * 100 µG

Akce

Katalogové číslo: (GENOBT927-220E)

Dodavatel: G-Biosciences

Popis: This orbital shaker is compact and well designed.

Měrná jednotka: 1 * 1 KS

New Product Akce

Katalogové číslo: (BE-408)

Dodavatel: G-Biosciences

Popis: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of four different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-407)

Dodavatel: G-Biosciences

Popis: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-415)

Dodavatel: G-Biosciences

Popis: Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-201)

Dodavatel: G-Biosciences

Popis: This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BKC-15)

Dodavatel: G-Biosciences

Popis: G-Biosciences' Sodium meta -Periodate is a mild oxidizing agent that converts carbohydrates to activated active aldehydes

Měrná jednotka: 1 * 5 g

Akce

Katalogové číslo: (786-232)

Dodavatel: G-Biosciences

Popis: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (786-246)

Dodavatel: G-Biosciences

Popis: Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: G-Biosciences

Popis: For the rapid purification of soluble, 6X His or GST tagged proteins.

Dodavatel: G-Biosciences

Popis: Hydrogenuhličitan sodný

Dodavatel: G-Biosciences

Popis: L(+)-Kyselina askorbová

Dodavatel: G-Biosciences

Popis: These disposable spin columns are designed for protein purification. These spin columns are uniquely designed, with a snap off end converting to a closure for the column for easy manipulation and use.

Dodavatel: G-Biosciences

Popis: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Katalogové číslo: (786-409)

Dodavatel: G-Biosciences

Popis: LabSafe™ Nucleic Acid Stain is a safe nucleic acid stain for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels.

Měrná jednotka: 1 * 1 mL

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