Hledali jste: G Bioscienes

Katalogové číslo: (BE-401)

Dodavatel: G-Biosciences

Popis: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (786-651)

Dodavatel: G-Biosciences

Popis: β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.

Měrná jednotka: 1 * 500 Assays

Akce

Katalogové číslo: (786-627)

Dodavatel: G-Biosciences

Popis: For improved dialysis time and rates.

Měrná jednotka: 1 * 50 mL

Akce

Dodavatel: G-Biosciences

Popis: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Dodavatel: G-Biosciences

Popis: The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

Dodavatel: G-Biosciences

Popis: EDTA disodium salt is a reversible metalloprotease inhibitor that is used for chelating metal ions.

Bezpečnostní list

Katalogové číslo: (P315)

Dodavatel: G-Biosciences

Popis: Chlorid draselný

Měrná jednotka: 1 * 100 g

Akce

Dodavatel: G-Biosciences

Popis: A proprietary mixture of Formaldehyde, EDTA and Blue Dextran that is designed for use in loading DNA samples onto denaturing polyacrylamide gels.

Katalogové číslo: (786-791)

Dodavatel: G-Biosciences

Popis: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (786-790)

Dodavatel: G-Biosciences

Popis: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (BE-502)

Dodavatel: G-Biosciences

Popis: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: G-Biosciences

Popis: GET™ DNA Template is a spin column format kit suitable for the preparation of DNA templates from blood, cells, fungal and animal tissue samples. The isolated genomic DNA is suitable for PCR and other downstream applications.

Katalogové číslo: (P317)

Dodavatel: G-Biosciences

Popis: Hydroxid draselný

Měrná jednotka: 1 * 100 g

Akce

Katalogové číslo: (786-453)

Dodavatel: G-Biosciences

Popis: The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (786-488)

Dodavatel: G-Biosciences

Popis: Dihydrogenfosforečnan draselný 1 mol/l vodný roztok

Měrná jednotka: 1 * 1 L

Akce

Katalogové číslo: (ML-04)

Dodavatel: G-Biosciences

Popis: GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Měrná jednotka: 1 * 150 µG

Akce