Hledali jste: G Bioscienes

Katalogové číslo: (RC-017)

Dodavatel: G-Biosciences

Popis: Peroxodisíran amonný, powder for electrophoresis

Měrná jednotka: 1 * 100 g

Akce

Katalogové číslo: (BE-308)

Dodavatel: G-Biosciences

Popis: Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: G-Biosciences

Popis: The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Dodavatel: G-Biosciences

Popis: The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Dodavatel: G-Biosciences

Popis: Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Katalogové číslo: (786-016)

Dodavatel: G-Biosciences

Popis: Dodecylsíran sodný 200 g/l vodný roztok

Měrná jednotka: 1 * 500 mL

Akce

Dodavatel: G-Biosciences

Popis: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Katalogové číslo: (786-793)

Dodavatel: G-Biosciences

Popis: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

Měrná jednotka: 1 * 5 mL

Akce

Dodavatel: G-Biosciences

Popis: The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

Dodavatel: G-Biosciences

Popis: HOOK™-Biotin-PDA, 50 mg

Dodavatel: G-Biosciences

Popis: These cross-linking agents contain at least two reactive groups that are reactive towards numerous groups, including sulphydryls, amines and carbohydrates, and create chemical covalent bonds between two or more molecules. Functional groups that can be targeted with cross-linking agents are primary amines, carboxyls, sulfhydryls, carbohydrates and carboxylic acids. Protein molecules have many of these functional groups and therefore proteins and peptides can be readily conjugated using cross-linking agents.

Katalogové číslo: (BE-104)

Dodavatel: G-Biosciences

Popis: This DNA fingerprinting kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a pseudo-crime scene.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: G-Biosciences

Popis: Glutathione resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8 mg GST/ml resin. Supplied as slurry in 20% ethanol.

Dodavatel: G-Biosciences

Popis: Brij® 58

Katalogové číslo: (786-681)

Dodavatel: G-Biosciences

Popis: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: G-Biosciences

Popis: Tri-Xtract™ is designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridisation, <i>in vitro</i> translation, RNase protection assays and poly (A+) selection.