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Voda for biotechnology nuclease-free, 0.2 µm filtered

Dodavatel: VWR Chemicals

Popis: Nuclease-free water treated with diethylpyrocarbonate (DEPC) for nucleic acid applications.

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Mag-Bind® Plant DNA DS 96 kit

Dodavatel: OMEGA BIO-TEK

Popis: The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

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TriLink® 5-Methylcytidine-5'-Triphosphate

Dodavatel: TriLink BioTechnologies

Popis: This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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VWR®, Taq DNA polymerase glycerol free

Dodavatel: VWR Chemicals

Popis: VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

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VWR®, Nucleic acid loading dyes

Katalogové číslo: (E190-5ML)

Dodavatel: VWR Chemicals

Popis: Designed to optimise loading nucleic acids in agarose gels, the loading dye imparts colour to the sample to facilitate the loading process and increase the density of the sample to ensure efficient sample distribution into each well. The dye migrates independently from the sample, allowing estimation of the migration of nucleic acids.

Měrná jednotka: 1 * 5 mL

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Succinimidyl ester acridine orange DNA/RNA binding dye

Katalogové číslo: (40074.)

Dodavatel: Biotium

Popis: The amine reactive succinimidyl ester of green fluorescent AO (acridine orange) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups. The conjugates then are able to complex with nucleic acids, resulting in green fluorescence nucleic acid conjugate adduct, making them potentially useful for studies of nucleic acid binding to various biomolecules, such as DNA-binding proteins.

Měrná jednotka: 1 * 5 mg

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AMPIGENE® dNTP Mix

Katalogové číslo: (ENZOENZNUC1020500)

Dodavatel: ENZO LIFE SCIENCES

Popis: Ultra-pure, stable, and suitable for a variety of PCR applications.

Měrná jednotka: 1 * 500 µl

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DMAO 2 mM in DMSO used in nuclear staining

Katalogové číslo: (40012.)

Dodavatel: Biotium

Popis: DMAO, 2 mM in DMSO is a green-fluorescent nucleic acid dye for staining live bacteria.

Měrná jednotka: 1 * 1 mL

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E.Z.N.A.® Plasmid Maxi kit

Dodavatel: OMEGA BIO-TEK

Popis: The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription invitro, and other applications.

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