Hledali jste: Haloenol+lactone+suicide+substrate

Katalogové číslo: (BOSSBS-1135R-CY3)

Dodavatel: Bioss

Popis: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Měrná jednotka: 1 * 100 µl

Akce

Dodavatel: Apollo Scientific

Popis: Histochemical substrate for esterase

Katalogové číslo: (BOSSBS-5935R-A555)

Dodavatel: Bioss

Popis: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex. The BCR(KLHL7) complex acts by mediating ubiquitination and subsequent degradation of substrate proteins. Probably mediates 'Lys-48'-linked ubiquitination.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (BOSSBS-5935R-A488)

Dodavatel: Bioss

Popis: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex. The BCR(KLHL7) complex acts by mediating ubiquitination and subsequent degradation of substrate proteins. Probably mediates 'Lys-48'-linked ubiquitination.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (BOSSBS-5104R-HRP)

Dodavatel: Bioss

Popis: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (BOSSBS-3359R-A350)

Dodavatel: Bioss

Popis: MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (BOSSBS-3263R-FITC)

Dodavatel: Bioss

Popis: MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.

Měrná jednotka: 1 * 100 µl

Akce

Dodavatel: ENZO LIFE SCIENCES

Popis: Substrate for LTA₄ hydrolase and LTC₄ synthase.

Dodavatel: Abcam

Popis: Chromogenic β-galactosidase substrate yielding a yellow end product upon cleavage.

Katalogové číslo: (ENZOBMLAW97850005)

Dodavatel: ENZO LIFE SCIENCES

Popis: This fluorogenic peptide is often the substrate of choice for assaying the trypsin-like activity of purified proteasomes. Benefits include a low Km, high specific activity, and the fact that the substrate is cleaved exclusively at the R-AMC bond.

Měrná jednotka: 1 * 5 mg

Akce

Katalogové číslo: (BOSSBS-3695R-CY7)

Dodavatel: Bioss

Popis: NTF97 is involved in nuclear protein import, either by associating itself with an adapter protein (for example, importin-alpha subunit which binds to nuclear localization signals (NLS) in cargo substrates), or by acting autonomously as a nuclear transport receptor (serves as NLS receptor, docking of the importin/substrate complex to the nuclear pore complex).

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (ENZOBMLAK7110001)

Dodavatel: ENZO LIFE SCIENCES

Popis: The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Apollo Scientific

Popis: X-GlcA is a substrate for b-Glucuronidase (GUS) encoded by the gusA gene. The substrate is used as a qualitative marker of specific GUS expression.

Dodavatel: ENZO LIFE SCIENCES

Popis: Hsp40/Hdj1 is a cytosolic co-chaperone belonging to the class DnaJ, named after its homolog in E. coli. Hsp40 functions in protein folding by binding nascent peptides and unfolded substrates and facilitating substrate interaction with Hsp70. Binding of Hsp40 to Hsp70 increases Hsp70 ATP hydrolysis and substrate binding, which is reversed by the action of nucleotide exchange factors (GrpE in E. coli). Repeated cycles of Hsp40/Hsp70 peptide binding and ATP hydrolysis prevent premature folding and aggregation, promoting protein maturation throughout the cell.

Katalogové číslo: (ENZOALX260120M005)

Dodavatel: ENZO LIFE SCIENCES

Popis: Substrate for MMP-2 and -9

Měrná jednotka: 1 * 5 mg

Akce

Katalogové číslo: (ENZOBMLKI1740005)

Dodavatel: ENZO LIFE SCIENCES

Popis: The deacetylated state of lysine-16 of histone H4 (H4-K16) is thought to be a critical factor in determining regions of chromatin silencing. Conversely, acetylation of lysine-16 can be associated with increased transcription, such as that due to dosage compensation from the X-chromosome of male Drosophila.

Měrná jednotka: 1 * 1 Vial

Akce