Hledali jste: DISTEK

Katalogové číslo: (BOSSBS-1135R-A350)

Dodavatel: Bioss

Popis: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (ENZOBMLP3150500)

Dodavatel: ENZO LIFE SCIENCES

Popis: Insulin receptor substrate

Měrná jednotka: 1 * 0,5 mg

Akce

Katalogové číslo: (BOSSBS-4210R-A680)

Dodavatel: Bioss

Popis: hydrolyses acyl-CoA thioesters (in vitro). Has a preference for substrates with medium chain length (C1-C14). Inactive towards substrates with C18 or C2 aliphatic chains. Its physiological substrate is not known.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (ENZOBMLP2680001)

Dodavatel: ENZO LIFE SCIENCES

Popis: LRRK substrate

Měrná jednotka: 1 * 1 mg

Akce

Katalogové číslo: (BOSSBS-4210R-A488)

Dodavatel: Bioss

Popis: Hydrolyzes acyl-CoA thioesters (in vitro). Has a preference for substrates with medium chain length (C1-C14). Inactive towards substrates with C18 or C2 aliphatic chains. Its physiological substrate is not known.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (ENZOBMLP2320001)

Dodavatel: ENZO LIFE SCIENCES

Popis: AMPK substrate

Měrná jednotka: 1 * 1 mg

Akce

Dodavatel: ENZO LIFE SCIENCES

Popis: Substrate for caspase-1 and -4

Dodavatel: ENZO LIFE SCIENCES

Popis: Caspase-8 substrate

Dodavatel: Biotium

Popis: Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

Katalogové číslo: (BOSSBS-4210R-HRP)

Dodavatel: Bioss

Popis: Hydrolyzes acyl-CoA thioesters (in vitro). Has a preference for substrates with medium chain length (C1-C14). Inactive towards substrates with C18 or C2 aliphatic chains. Its physiological substrate is not known.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (ENZOBMLP4480005)

Dodavatel: ENZO LIFE SCIENCES

Popis: DPP substrate

Měrná jednotka: 1 * 5 mg

Akce

Katalogové číslo: (ENZOBMLP1640001)

Dodavatel: ENZO LIFE SCIENCES

Popis: VHR substrate

Měrná jednotka: 1 * 1 mg

Akce

Katalogové číslo: (ENZOALX260130M005)

Dodavatel: ENZO LIFE SCIENCES

Popis: Cathepsin substrate

Měrná jednotka: 1 * 5 mg

Akce

Dodavatel: ENZO LIFE SCIENCES

Popis: Caspase and granzyme substrate

Dodavatel: ENZO LIFE SCIENCES

Popis: Caspase-9 substrate

Katalogové číslo: (ENZOBMLP1270001)

Dodavatel: ENZO LIFE SCIENCES

Popis: Reference peptide for OmniMMPTM fluorogenic substrate (BML-P126) and TACE (ADAM17) fluorogenic substrate (BML-P132). Ex.: 328 nm, Em.: 393 nm.

Měrná jednotka: 1 * 1 mg

Akce