Hledali jste: Haloenol+lactone+suicide+substrate

Katalogové číslo: (ENZOBMLUW05001000)

Dodavatel: ENZO LIFE SCIENCES

Popis: SUMO1-AMCA is a synthetic AMCA-peptide substrate based on the C-terminal residues of SUMO1 and is a useful fluorogenic substrate for deSUMOylating enzymes, including SENP1 (BML-UW9760).

Měrná jednotka: 1 * 1 mg

Akce

Katalogové číslo: (ENZOALX260130M005)

Dodavatel: ENZO LIFE SCIENCES

Popis: Cathepsin substrate

Měrná jednotka: 1 * 5 mg

Akce

Dodavatel: ENZO LIFE SCIENCES

Popis: Caspase and granzyme substrate

Dodavatel: ENZO LIFE SCIENCES

Popis: Caspase-9 substrate

Katalogové číslo: (ENZOALX155023M001)

Dodavatel: ENZO LIFE SCIENCES

Popis: Specific PKC substrate

Měrná jednotka: 1 * 1 mg

Akce

Dodavatel: ENZO LIFE SCIENCES

Popis: Caspase-1 substrate

Katalogové číslo: (ENZOBMLP1380025)

Dodavatel: ENZO LIFE SCIENCES

Popis: Cathepsin substrate

Měrná jednotka: 1 * 25 mg

Akce

Katalogové číslo: (ENZOBMLP1620001)

Dodavatel: ENZO LIFE SCIENCES

Popis: ACE substrate

Měrná jednotka: 1 * 1 mg

Akce

Dodavatel: G-Biosciences

Popis: The femtoELISA™ kits utilise a non animal protein blocker, NAP-BLOCKER™ that minimises cross-reactivity with researcher's antigens and antibodies. For the detection of HRP, an improved, ultra sensitive, non volatile, stable colorimetric substrate based on tetramethyl benzidine (TMB) is supplied. Reagent does not require hydrogen peroxide that can have detrimental effects on many biological factors, which may ultimately affect the assays.

Certifikáty

Dodavatel: ENZO LIFE SCIENCES

Popis: Caspase-2 substrate

Katalogové číslo: (ENZOBMLP2680001)

Dodavatel: ENZO LIFE SCIENCES

Popis: LRRK substrate

Měrná jednotka: 1 * 1 mg

Akce

Katalogové číslo: (ENZOBMLP3150500)

Dodavatel: ENZO LIFE SCIENCES

Popis: Insulin receptor substrate

Měrná jednotka: 1 * 0,5 mg

Akce

Dodavatel: Apollo Scientific

Popis: Optical brightner. Highly effective in polymer substrates such as engineering plastics (polyesters, polycarbonate, polyamides and acrylics, thermoplastic polyurethane, polyvinylchloride, styrene homo- and copolymers, polyolefins), adhesives and other organic substrates.

Katalogové číslo: (ENZOBMLP2320001)

Dodavatel: ENZO LIFE SCIENCES

Popis: AMPK substrate

Měrná jednotka: 1 * 1 mg

Akce

Katalogové číslo: (BOSSBS-1135R-A555)

Dodavatel: Bioss

Popis: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (BOSSBS-1135R-HRP)

Dodavatel: Bioss

Popis: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Měrná jednotka: 1 * 100 µl

Akce