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Restriction enzymes, Dryzymes®

Dodavatel: EDVOTEK
Popis: The three most frequently used restriction enzymes are Eco RI, Bam HI and Hind III. Each enzyme catalyses cleavage at the defined base sequence. All enzymes are lyophilised and contain 1500 units. One unit is defined as the amount of enzyme required to digest 1,0 µg of lambda DNA in 60 minutes at 37 °C in a total reaction mixture of 50 µl.

Product Image-EDVO225

Experimentation kits, DNA fingerprinting using restriction enzymes

Katalogové číslo: (EDVO225)
Dodavatel: EDVOTEK
Popis: This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.
Měrná jednotka: 1 * 1 KIT

Bezpečnostní list


Product Image-BE-203

Experimentation kits, antibiotic sensitivity and bacteria screening

Katalogové číslo: (BE-203)
Dodavatel: G-Biosciences
Popis: Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.
Měrná jednotka: 1 * 1 KIT
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What's in my lunch? quantitative milk allergy ELISA kit

Katalogové číslo: (EDVO266)
Dodavatel: EDVOTEK
Popis: Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.
Měrná jednotka: 1 * 1 KIT
Product Image-BE-312

Experimentation kits, expression of a recombinant protein

Katalogové číslo: (BE-312)
Dodavatel: G-Biosciences
Popis: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).
Měrná jednotka: 1 * 1 KIT
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ReadyPour™ Luria Broth (LB) agar base

Katalogové číslo: (EDVO615)
Dodavatel: EDVOTEK
Popis: Educational Materials, Microbiology, Aplikace: Mikrobiologie
Měrná jednotka: 1 * 1 mL
Product Image-763-0002

Experimentation kits, Quick PCR

Katalogové číslo: (763-0002)
Dodavatel: EDVOTEK
Popis: This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.
Měrná jednotka: 1 * 1 KIT
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Kits: DNA paternity test, Ready-to-Load™

Dodavatel: EDVOTEK
Popis: This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents.

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Transformation with blue and green fluorescent proteins

Katalogové číslo: (EDVO222)
Dodavatel: EDVOTEK
Popis: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.
Měrná jednotka: 1 * 1 KS

Bezpečnostní list


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Experimentation kits, exploring biotechnology with green fluorescent protein (GFP)

Katalogové číslo: (EDVO303)
Dodavatel: EDVOTEK
Popis: Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.
Měrná jednotka: 1 * 1 KIT
Product Image-EDVO255

Purification and size determination of green and blue fluorescent proteins

Katalogové číslo: (EDVO255)
Dodavatel: EDVOTEK
Popis: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
Měrná jednotka: 1 * 1 KIT
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Experimentation kits, in search of the 'Kissing Disease'

Katalogové číslo: (EDVO274)
Dodavatel: EDVOTEK
Popis: Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.
Měrná jednotka: 1 * 1 KS

Bezpečnostní list


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Experimentation kits, southern blot analysis

Katalogové číslo: (EDVO207)
Dodavatel: EDVOTEK
Popis: This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.
Měrná jednotka: 1 * 1 KS
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Experimentation kits, analysis and comparision of mammalian cell types

Katalogové číslo: (EDVO986)
Dodavatel: EDVOTEK
Popis: Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.
Měrná jednotka: 1 * 1 KS
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Experimentation kits, antigen-antibody interaction: the ouchterlony procedure

Katalogové číslo: (EDVO270)
Dodavatel: EDVOTEK
Popis: This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.
Měrná jednotka: 1 * 1 KS
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Experimentation kits, simulation of HIV detection by ELISA

Katalogové číslo: (EDVO271)
Dodavatel: EDVOTEK
Popis: An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.
Měrná jednotka: 1 * 1 KS
Cena na vyžádání
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Daná chemikálie je regulována dle platné legislativy a bude vyžadováno vyplnění formuláře. Jeho včasným vyplněním uspíšíte dodání produktu.
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Tento produkt již není k dispozici. Podobné produkty můžete vyhledat pomocí VWR katalogových čísel nebo čísel dodavatele uvedených výše. Pokud potřebujete další asistenci, zavolejte na telefonní číslo zákaznického servisu VWR 321 570 321.
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