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Katalogové číslo: (EDVO314)

Dodavatel: EDVOTEK

Popis: Suppressor genes, such as p53, are essential for cell functions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations of genes can either be inherited or accumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO333)

Dodavatel: EDVOTEK

Popis: Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-406)

Dodavatel: G-Biosciences

Popis: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-409)

Dodavatel: G-Biosciences

Popis: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of three functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: MEMMERT

Popis: Příslušenství pro řady VOcool a VO

Katalogové číslo: (BE-502)

Dodavatel: G-Biosciences

Popis: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO243)

Dodavatel: EDVOTEK

Popis: Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Měrná jednotka: 1 * 1 KS

Bezpečnostní list Akce

Katalogové číslo: (EDVO113)

Dodavatel: EDVOTEK

Popis: This experiment introduces chromatographic theory and methods of thin layer chromatography (TLC). A mixture of dyes are separated on a cellulose-based TLC plate using different solvent systems.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (BE-415)

Dodavatel: G-Biosciences

Popis: Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (552-0089)

Dodavatel: MACHEREY-NAGEL

Popis: An ideal introductory kit for the science education sector. The kit contains all the the chemicals, plates and accessories for students to perform separations of dyes including: anthaquinone dye mixtures, lipophilic dye mixtures and food dye mixtures. The TLC plates are polyester sheet based with either silica, cellulose or alumina as coating.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO108)

Dodavatel: EDVOTEK

Popis: This experiment introduces chromatographic separation to the class and shows students how dyes of different colours separate on the basis of their size and shape. Columns may be rinsed and reused.

Měrná jednotka: 1 * 1 KS

Bezpečnostní list Akce

Katalogové číslo: (MANA814400)

Dodavatel: MACHEREY-NAGEL

Popis: Advanced kit for science education.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (552-0282)

Dodavatel: MACHEREY-NAGEL

Popis: Materials kit for scientific education.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (BE-507)

Dodavatel: G-Biosciences

Popis: A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilised on a chromatographic resin. The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction. In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilised in research laboratories.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: G-Biosciences

Popis: Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Katalogové číslo: (BE-301)

Dodavatel: G-Biosciences

Popis: Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.

Měrná jednotka: 1 * 1 KIT

Akce