Dodavatel: OMEGA BIO-TEK

Popis: Isolate DNA from plant or fungal sample with organic solvents using spin columns.

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Dodavatel: OMEGA BIO-TEK

Popis: The Mag-Bind® cfDNA Kit is designed for rapid and reliable isolation of circulating DNA from 500 to 4000 µl plasma or serum samples. The Mag-Bind® cfDNA Kit can be processed manually or with automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.

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Dodavatel: OMEGA BIO-TEK

Popis: E.Z.N.A.® M13 DNA kits are designed to purify up to 10 μg of single-stranded DNA from up to 3 ml of phage supernatant. Yields of single-stranded DNA obtained using E.Z.N.A.® M13 DNA kits are around 3 to 10 μg and reproducible when the isolations are performed from the same culture. Kits are also available in E-Z 96® DNA plate format.

Dodavatel: OMEGA BIO-TEK

Popis: The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

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Dodavatel: OMEGA BIO-TEK

Popis: Isolates total RNA from blood samples stored in special preservation reagents and PAXgene tubes using spin columns.

Dodavatel: OMEGA BIO-TEK

Popis: Isolate total cellular RNA and viral RNA from a wide variety of cells and tissues.

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Dodavatel: OMEGA BIO-TEK

Popis: The Mag-Bind® Universal Pathogen DNA 96 Kit allows rapid and reliable isolation of high-quality pathogen and host genomic DNA from tissue samples. Up to a 30 mg tissue sample is recommended and can be processed in less than 60 minutes. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.

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Dodavatel: OMEGA BIO-TEK

Popis: Hi-Bind® DNA mini columns are silica glass fibre columns that can bind up to 100 μg of genomic DNA or 35 μg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation.

Dodavatel: OMEGA BIO-TEK

Popis: Isolate DNA from molluscs and insects using spin columns.

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Dodavatel: OMEGA BIO-TEK

Popis: Sequentially isolate DNA and RNA in two separate eluates from the same FFPE sample.

Dodavatel: OMEGA BIO-TEK

Popis: The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.

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Dodavatel: OMEGA BIO-TEK

Popis: Selektivní štěpení proteázou uvolňuje z FFPE vzorků mikrogramová množství DNA. Purifikované nukleové kyseliny, ačkoliv vysoce fragmentované, jsou vhodné pro širokou škálu následných genomových analýz a analýz genové exprese. Soupravy E.Z.N.A.® obsahující centrifugační kolonky jsou ideální pro nízký počet vzorků, zatímco Mag-Bind® soupravy fungující na principu magnetických kuliček jsou navrženy speciálně pro uživatele s vysokým počtem vzorků s možností automatizace.

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Dodavatel: OMEGA BIO-TEK

Popis: Souprava E.Z.N.A.® Water DNA Kit je navržena pro izolaci vysoce čisté buněčné DNA z vodních vzorků obsahujících huminové kyseliny a inhibitory PCR. Tato souprava je velice účinná při izolaci DNA z bakterií, u kterých se těžko provádi lyze, z hub a z řas, které se nachází ve vodě z vodovodního řádu, jezerech, řekách a v kanalizaci. DNA purifikovaná pomocí soupravy E.Z.N.A.® Water DNA Kit je připravena pro následné aplikace, jako jsou PCR, Southern blotting a anylýza SNP.

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Dodavatel: Corning

Popis: The AxyPrep™ FragmentSelect-I Kit is optimized for Illumina next generation sequencing platforms and Life Technologies (SOLiD) fragment size selection needs to simplify the library construction workflow. The kit is designed for fragment size selection needs in the Roche/454’s DNA Genome sequencer library construction workflow.

Dodavatel: OMEGA BIO-TEK

Popis: The E.Z.N.A.® Total RNA kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the E.Z.N.A.® Total RNA kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the E.Z.N.A.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and<i> in vitro</i> translation.

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Dodavatel: OMEGA BIO-TEK

Popis: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.