Hledali jste: Aktivity pro výuku biochemie

Katalogové číslo: (BE-403)

Dodavatel: G-Biosciences

Popis: Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-406)

Dodavatel: G-Biosciences

Popis: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-409)

Dodavatel: G-Biosciences

Popis: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of three functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-408)

Dodavatel: G-Biosciences

Popis: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of four different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-407)

Dodavatel: G-Biosciences

Popis: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-308)

Dodavatel: G-Biosciences

Popis: Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-312)

Dodavatel: G-Biosciences

Popis: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-314)

Dodavatel: G-Biosciences

Popis: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-318)

Dodavatel: G-Biosciences

Popis: The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.

Měrná jednotka: 1 * 1 KIT

Akce