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Popis: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Dodavatel: TriLink BioTechnologies

Popis: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Dodavatel: TriLink BioTechnologies

Popis: T7 RNA Polymerase is a wild-type enzyme to synthesize RNA from DNA templates by <em>in vitro</em> transcription.

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Popis: Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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Popis: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

Dodavatel: TriLink BioTechnologies

Popis: This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

Dodavatel: TriLink BioTechnologies

Popis: Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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Dodavatel: TriLink BioTechnologies

Popis: β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

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Popis: <p>Cas9 Nickase mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.</p>

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Popis: CleanScribe™ RNA Polymerase is a novel enzyme that reduces double-stranded RNA (dsRNA) formation in IVT RNA synthesis.

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Popis: The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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Popis: EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko <em>et al.</em> showed that transfection of EPO mRNA <em>in vivo</em> resulted in significant increases of both reticulocyte counts and hematocrits.

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Dodavatel: TriLink BioTechnologies

Popis: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Popis: Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.

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Popis: CleanCap® Reagent AU is designed for the co-transcriptional capping of self-replicating or self-amplifying (saRNA) to produce an optimal Cap 1.

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Popis: Inorganic Pyrophosphatase (iPPase) is an enzyme to remove pyrophosphate in IVT to drive the reaction forward.

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