Hledali jste: BIOWEST

Dodavatel: Biowest

Popis: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

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Dodavatel: Biowest

Popis: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

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Katalogové číslo: (L0014-100)

Dodavatel: Biowest

Popis: Glutamine penicillin streptamycin acts against gram-negative and gram-positive bacteria.

Měrná jednotka: 1 * 100 mL

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Dodavatel: Biowest

Popis: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

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Dodavatel: Biowest

Popis: Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.

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Dodavatel: Biowest

Popis: Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

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Katalogové číslo: (X0550-100)

Dodavatel: Biowest

Popis: L(+)-Glutamin 0.2 mol/l in aqueous solution

Měrná jednotka: 1 * 100 mL

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Dodavatel: Biowest

Popis: McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

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Dodavatel: Biowest

Popis: Antibiotic-antimycotic used in tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi. Penicillin acts by inhibiting bacterial cell wall synthesis.

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Dodavatel: Biowest

Popis: Cell culture water is high-quality water suitable for use as a solvent in the preparation of cell culture media and laboratory reagents.

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Katalogové číslo: (L0018-100)

Dodavatel: Biowest

Popis: Penicillin-Streptomycin acts against gram-negative, gram-positive bacteria and mycoplasma.

Měrná jednotka: 1 * 100 mL

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Katalogové číslo: (L0207-500)

Dodavatel: Biowest

Popis: Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, <i>Drosophila melanogaster</i>. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

Měrná jednotka: 1 * 500 mL

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Katalogové číslo: (L0950-100)

Dodavatel: Biowest

Popis: Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.

Měrná jednotka: 1 * 100 mL

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Dodavatel: Biowest

Popis: RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

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Dodavatel: Biowest

Popis: Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

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Dodavatel: Biowest

Popis: Antibiotic G418 is an aminoglycoside antibiotic similar in structure to gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. The optimal concentration for selection and maintenance must be determined for each cell line.

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