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Popis: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.
Katalogové číslo: 786-077
Měrná jednotka: 1 * 40 KS
Dodavatel: G-Biosciences


Popis: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
Katalogové číslo: 786-241
Měrná jednotka: 1 * 1 KIT
Dodavatel: G-Biosciences


Popis: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
Katalogové číslo: PIER90067
Měrná jednotka: 1 * 1 KIT
Dodavatel: Thermo Fisher Scientific


Popis: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.
Katalogové číslo: 786-156
Měrná jednotka: 1 * 10 KS
Dodavatel: G-Biosciences


Popis: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
Katalogové číslo: ENZOBMLKI1050300
Měrná jednotka: 1 * 300 µl
Dodavatel: ENZO LIFE SCIENCES


Popis: Neuronal culture medium, Pierce
Katalogové číslo: PIER88285
Měrná jednotka: 1 * 1 KIT
Dodavatel: Thermo Fisher Scientific


Popis: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
Katalogové číslo: PIER90054
Měrná jednotka: 1 * 50 µG
Dodavatel: Thermo Fisher Scientific

Popis: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
Katalogové číslo: PIER90056
Měrná jednotka: 1 * 100 µG
Dodavatel: Thermo Fisher Scientific


Popis: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
Katalogové číslo: 786-599
Měrná jednotka: 1 * 5 mL
Dodavatel: G-Biosciences


Popis: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
Katalogové číslo: PIER90053
Měrná jednotka: 1 * 2 µG
Dodavatel: Thermo Fisher Scientific

Popis: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.
Katalogové číslo: 786-214
Měrná jednotka: 1 * 10 KS
Dodavatel: G-Biosciences

Popis: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.
Katalogové číslo: 786-090
Měrná jednotka: 1 * 8 KS
Dodavatel: G-Biosciences


Popis: The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.
Katalogové číslo: PIER84841
Měrná jednotka: 1 * 10 µG
Dodavatel: Thermo Fisher Scientific


Popis: Pierce™ C18 spin tips, for elution of up to 10 µg peptides per 20 µl sample for MS
Katalogové číslo: PIER84850
Měrná jednotka: 1 * 1 KIT
Dodavatel: Thermo Fisher Scientific


Popis: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
Katalogové číslo: 786-428
Měrná jednotka: 1 * 0,25 mL
Dodavatel: G-Biosciences


Popis: Immobilised Anti-TMT™ antibody resin
Katalogové číslo: PIER90114
Měrná jednotka: 1 * 50 mL
Dodavatel: Thermo Fisher Scientific


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