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Popis: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Katalogové číslo: 786-077

Měrná jednotka: 1 * 40 KS

Dodavatel: G-Biosciences

Akce

Popis: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

Katalogové číslo: 786-241

Měrná jednotka: 1 * 1 KIT

Dodavatel: G-Biosciences

Akce

Popis: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.

Katalogové číslo: PIER90067

Měrná jednotka: 1 * 1 KIT

Dodavatel: Thermo Fisher Scientific

Akce

Popis: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Katalogové číslo: 786-156

Měrná jednotka: 1 * 10 KS

Dodavatel: G-Biosciences

Akce

Popis: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).

Katalogové číslo: ENZOBMLKI1050300

Měrná jednotka: 1 * 300 µl

Dodavatel: ENZO LIFE SCIENCES

Akce

Popis: Neuronal culture medium, Pierce

Katalogové číslo: PIER88285

Měrná jednotka: 1 * 1 KIT

Dodavatel: Thermo Fisher Scientific

Akce

Popis: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.

Katalogové číslo: PIER90054

Měrná jednotka: 1 * 50 µG

Dodavatel: Thermo Fisher Scientific

Bezpečnostní list Akce

Popis: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.

Katalogové číslo: PIER90056

Měrná jednotka: 1 * 100 µG

Dodavatel: Thermo Fisher Scientific

Akce

Popis: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.

Katalogové číslo: 786-599

Měrná jednotka: 1 * 5 mL

Dodavatel: G-Biosciences

Akce

Popis: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

Katalogové číslo: PIER90053

Měrná jednotka: 1 * 2 µG

Dodavatel: Thermo Fisher Scientific

Bezpečnostní list Akce

Popis: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Katalogové číslo: 786-214

Měrná jednotka: 1 * 10 KS

Dodavatel: G-Biosciences

Certifikáty Akce

Popis: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Katalogové číslo: 786-090

Měrná jednotka: 1 * 8 KS

Dodavatel: G-Biosciences

Akce

Popis: The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.

Katalogové číslo: PIER84841

Měrná jednotka: 1 * 10 µG

Dodavatel: Thermo Fisher Scientific

Akce

Popis: Pierce™ C18 spin tips, for elution of up to 10 µg peptides per 20 µl sample for MS

Katalogové číslo: PIER84850

Měrná jednotka: 1 * 1 KIT

Dodavatel: Thermo Fisher Scientific

Akce

Popis: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.

Katalogové číslo: 786-428

Měrná jednotka: 1 * 0,25 mL

Dodavatel: G-Biosciences

Akce

Popis: Immobilised Anti-TMT™ antibody resin

Katalogové číslo: PIER90114

Měrná jednotka: 1 * 50 mL

Dodavatel: Thermo Fisher Scientific

Akce