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Product Image-M1682

Maxima™ H minus cDNA synthesis master mix, with dsDNase

Dodavatel: Thermo Fisher Scientific
Popis: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Product Image-EP0743

Reverse transcriptase, Maxima™

Dodavatel: Thermo Fisher Scientific
Popis: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Product Image-27-9261-01

First-strand cDNA Synthesis kit

Katalogové číslo: (27-9261-01)
Dodavatel: Cytiva
Popis: Designed to generate full length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for <i>in vitro</i> amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.
Měrná jednotka: 1 * 55 Tests
Product Image-733-1098

RT-PCR kits, Verso™

Dodavatel: Thermo Fisher Scientific
Popis: The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.

Product Image-K1621

RevertAid™ First strand cDNA synthesis kit

Dodavatel: Thermo Fisher Scientific
Popis: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

Product Image-K1682

Maxima™ H minus first strand cDNA synthesis kits

Dodavatel: Thermo Fisher Scientific
Popis: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

Image Unavailable-ICNA11NTPMX100

dNTP mix, MP Biomedicals

Dodavatel: MP Biomedicals
Popis: This dNTP mix with dATP, dCTP, dGTP, dTTP is suitable for use in PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, DNA sequencing and labeling.

Product Image-K1632

RevertAid™ H minus first strand cDNA synthesis kits

Dodavatel: Thermo Fisher Scientific
Popis: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Product Image-EP0452

RevertAid™ H Minus Reverse Transcriptase

Dodavatel: Thermo Fisher Scientific
Popis: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Product Image-AATB17200

ReadiUse™ dNTP Mix *10 mM* (5 ml)

Katalogové číslo: (AATB17200)
Dodavatel: AAT BIOQUEST
Popis: Our Ready-to Use Solution of deoxynucleotide triphosphates (dNTPs) have been extensively tested and verified for use in a wide variety of molecular biology applications, including PCR, real-time PCR, high-fidelity PCR, long PCR, cDNA synthesis, RT-PCR, RCA, MDA, DNA labelling, DNA sequencing, <i>in vitro</i> transcription, siRNA synthesis, and RNA amplification.
Měrná jednotka: 1 * 5 mL
Product Image-27-9264-01

PCR beads, Ready-To-Go™ You-Prime First-Strand Beads

Katalogové číslo: (27-9264-01)
Dodavatel: Cytiva
Popis: Ready-To-Go™ You-Prime First-Strand beads are preformulated, single-dose reaction beads prepackaged in thin-walled 0,5 ml tubes compatible with most thermal cyclers. Ready-To-Go™ You-Prime First-Strand beads are used for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.
Měrná jednotka: 1 * 50 Tests

Bezpečnostní list


Product Image-EP0442

RevertAid™ Reverse Transcriptase

Dodavatel: Thermo Fisher Scientific
Popis: RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Image Unavailable-K1612

First strand cDNA synthesis kit

Katalogové číslo: (K1612)
Dodavatel: Thermo Fisher Scientific
Popis: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.
Měrná jednotka: 1 * 1 KIT
Image Unavailable-K0233

Maxima™ Probe qPCR Master Mix (2X)

Dodavatel: Thermo Fisher Scientific
Popis: The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Product Image-K0223

Maxima™ SYBR green/ROX QPCR master mix (2X)

Dodavatel: Thermo Fisher Scientific
Popis: The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Certifikáty

Product Image-K0251

Maxima™ SYBR® green QPCR master mix (2X), with ROX

Dodavatel: Thermo Fisher Scientific
Popis: The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Cena na vyžádání
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Daná chemikálie je regulována dle platné legislativy a bude vyžadováno vyplnění formuláře. Jeho včasným vyplněním uspíšíte dodání produktu.
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
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Původní produkt již není k dispozici. Zobrazen je náhradní produkt.
Tento produkt již není k dispozici. Podobné produkty můžete vyhledat pomocí VWR katalogových čísel nebo čísel dodavatele uvedených výše. Pokud potřebujete další asistenci, zavolejte na telefonní číslo zákaznického servisu VWR 321 570 321.
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