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Product Image-K1232

PCR cloning kit, CloneJET

Dodavatel: Thermo Fisher Scientific
Popis: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Product Image-EL0016

T4 DNA ligase

Katalogové číslo: (EL0016)
Dodavatel: Thermo Fisher Scientific
Popis: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
Měrná jednotka: 1 * 1 KIT

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Product Image-27-0361-01

T4 DNA ligase

Katalogové číslo: (27-0361-01)
Dodavatel: Cytiva
Popis: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
Měrná jednotka: 1 * 50 Tests
Product Image-K1261

LIC cloning and expression kits, aLICator™, Fermentas

Dodavatel: Thermo Fisher Scientific
Popis: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Product Image-SYGEGA1100-4X50

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Dodavatel: SGI - DNA Synthetic Genomics
Popis: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Image Unavailable-70573-3

Ligation premix, Clonables™

Katalogové číslo: (70573-3)
Dodavatel: Merck Millipore (Novagen)
Popis: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.
Měrná jednotka: 1 * 55 µl
Product Image-GA1100-10

DNA cloning kits, Gibson Assembly® HiFi 1 step kit

Dodavatel: SGI - DNA Synthetic Genomics
Popis: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

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Product Image-GA1200-10MM

Gibson Assembly® Ultra, Master mixes

Dodavatel: SGI - DNA Synthetic Genomics
Popis: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

Bezpečnostní list

Product Image-GA1200-50

Gibson Assembly® Ultra kit

Dodavatel: SGI - DNA Synthetic Genomics
Popis: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

Bezpečnostní list

Product Image-GA1100-10MM

Gibson Assembly® HiFi 1 Step, Master mixes

Dodavatel: SGI - DNA Synthetic Genomics
Popis: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

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Product Image-MFLX31608-20BLK

CPC® DrumQuik® PRO Drum Dispensing System Components

Dodavatel: Avantor Fluid Handling
Popis: Closed-system design ensures the safety of your environment and employees - at all phases of chemical handling.

Product Image-111-2496

Earmuffs, WS™ LiteCom Pro III Headset, Peltor™

Katalogové číslo: (111-2496)
Dodavatel: 3M
Popis: The 3M™ Peltor™ WS™ LiteCom Pro III Headset features an integrated, programmable analog/digital two-way radio, Bluetooth® Multipoint connectivity, noise-cancelling microphone, and level-dependent function for ambient listening. The headset not only helps protect against hazardous noise, but also makes it easy to communicate in noisy environments and maintain situational awareness.
Měrná jednotka: 1 * 1 KS
Image Unavailable-BOSSBS-11043R-A555

Anti-BEST4 Rabbit Polyclonal Antibody (Alexa Fluor® 555)

Katalogové číslo: (BOSSBS-11043R-A555)
Dodavatel: Bioss
Popis: Bestrophin-4 is a 473 amino acid member of the Bestrophin family of proteins. Members of the Bestrophin family are transmembrane proteins that contain a high percentage of aromatic residues, a conserved RFP (Arg-Phe-Pro) motif and they function as anion channels. Bestrophin-4 acts as a calcium-sensitive chloride channel located in the cell membrane. It is believed that Bestrophin-4 also acts as a channel for other physiologically significant anions, such as bicarbonate. Bestrophin-4 is predominantly expressed in the colon, but can be found at low levels in testis, placenta, trachea, spinal chord, lung and retina.
Měrná jednotka: 1 * 100 µl
Image Unavailable-BOSSBS-7613R-CY5

Anti-RNF144B Rabbit Polyclonal Antibody (Cy5®)

Katalogové číslo: (BOSSBS-7613R-CY5)
Dodavatel: Bioss
Popis: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.Tissue specificity: Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
Měrná jednotka: 1 * 100 µl
Image Unavailable-BOSSBS-11043R-CY5

Anti-BEST4 Rabbit Polyclonal Antibody (Cy5®)

Katalogové číslo: (BOSSBS-11043R-CY5)
Dodavatel: Bioss
Popis: Bestrophin-4 is a 473 amino acid member of the Bestrophin family of proteins. Members of the Bestrophin family are transmembrane proteins that contain a high percentage of aromatic residues, a conserved RFP (Arg-Phe-Pro) motif and they function as anion channels. Bestrophin-4 acts as a calcium-sensitive chloride channel located in the cell membrane. It is believed that Bestrophin-4 also acts as a channel for other physiologically significant anions, such as bicarbonate. Bestrophin-4 is predominantly expressed in the colon, but can be found at low levels in testis, placenta, trachea, spinal chord, lung and retina.
Měrná jednotka: 1 * 100 µl
Image Unavailable-PRSI91-088

Human Recombinant Bc1-w (from E. coli)

Katalogové číslo: (PRSI91-088)
Dodavatel: ProSci Inc.
Popis: Bcl-2-like protein 2 (BCL2L2) belongs to the Bcl-2 family. BCL2L2 is highly expressed in thebrain, spinal cord, testis, pancreas, heart, spleen, and mammary glands. BCL2L2 is a peripheral membrane protein containing three motifs, BH1, BH2 and BH4. The BH4 motif appears to be involved in the anti-apoptotic function. The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. BCL2L2 promotes cell survival and blocks dexamethasone-induced apoptosis. Furthermore, BCL2L2 mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
Měrná jednotka: 1 * 0,05 mg

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Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
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