Hledali jste: Haloenol+lactone+suicide+substrate

Katalogové číslo: (ENZOBMLP1420020)

Dodavatel: ENZO LIFE SCIENCES

Popis: Cathepsin K substrate

Měrná jednotka: 1 * 20 mg

Akce

Katalogové číslo: (BOSSBS-1135R-CY3)

Dodavatel: Bioss

Popis: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (ENZOBMLP2710500)

Dodavatel: ENZO LIFE SCIENCES

Popis: HAT substrate

Měrná jednotka: 1 * 0,5 mg

Akce

Katalogové číslo: (ENZOBMLP1380025)

Dodavatel: ENZO LIFE SCIENCES

Popis: Cathepsin substrate

Měrná jednotka: 1 * 25 mg

Akce

Katalogové číslo: (BOSSBS-1135R-A350)

Dodavatel: Bioss

Popis: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (ENZOBMLP2570001)

Dodavatel: ENZO LIFE SCIENCES

Popis: Protein kinase substrate

Měrná jednotka: 1 * 1 mg

Akce

Katalogové číslo: (ENZOBMLP1600500)

Dodavatel: ENZO LIFE SCIENCES

Popis: Calcineurin substrate

Měrná jednotka: 1 * 0,5 mg

Akce

Dodavatel: ENZO LIFE SCIENCES

Popis: Caspase-1 substrate

Dodavatel: Apollo Scientific

Popis: Fluorescent substrate for a-D-mannosidase.

Katalogové číslo: (ENZOBMLP2680001)

Dodavatel: ENZO LIFE SCIENCES

Popis: LRRK substrate

Měrná jednotka: 1 * 1 mg

Akce

Katalogové číslo: (ENZOBMLP2320001)

Dodavatel: ENZO LIFE SCIENCES

Popis: AMPK substrate

Měrná jednotka: 1 * 1 mg

Akce

Katalogové číslo: (ENZOBMLP4480005)

Dodavatel: ENZO LIFE SCIENCES

Popis: DPP substrate

Měrná jednotka: 1 * 5 mg

Akce

Katalogové číslo: (BOSSBS-10383R-A750)

Dodavatel: Bioss

Popis: The protein encoded by this gene is a substrate for the fibroblast growth factor receptor. It is found in peripheral plasma membrane and functions in linking FGF receptor stimulation to activators of Ras.

Měrná jednotka: 1 * 100 µl

Akce

Katalogové číslo: (ENZOBMLAK7110001)

Dodavatel: ENZO LIFE SCIENCES

Popis: The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (ENZOBMLP1270001)

Dodavatel: ENZO LIFE SCIENCES

Popis: Reference peptide for OmniMMPTM fluorogenic substrate (BML-P126) and TACE (ADAM17) fluorogenic substrate (BML-P132). Ex.: 328 nm, Em.: 393 nm.

Měrná jednotka: 1 * 1 mg

Akce

Dodavatel: ENZO LIFE SCIENCES

Popis: Endothelin-converting enzyme substrate