Hledali jste: Enzymatické+testy

Popis: Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for <i>E. coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

Katalogové číslo: ENZOENZ42831

Měrná jednotka: 1 * 25 Nmo

Dodavatel: ENZO LIFE SCIENCES

Akce

Popis: 2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve <em>in vivo</em> stability.

Katalogové číslo: SOLUN-1010-5

Měrná jednotka: 1 * 5 µmol

Dodavatel: TriLink BioTechnologies

New Product Akce

Popis: Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.

Katalogové číslo: ENZOENZ42502

Měrná jednotka: 1 * 25 Nmo

Dodavatel: ENZO LIFE SCIENCES

Akce

Popis: The fragmentation of genomic DNA by cellular nucleases during the later stages of apoptosis is also one of the most easily measured features of apoptotic cells. Nuclease activity generates DNA fragments ranging from ~300 bp to 50 bp in length, resulting in a typical DNA ‘laddering’ appearance when analyzed by agarose gel electrophoresis. These fragments have exposed 3’-hydroxyl (OH) ends which can be labeled with bromolated deoxyuridine triphosphates (Br-dUTP). An enzyme, terminal deoxynucleotidyl transferase (TdT), is used to catalyze the template-independent addition of Br-dUTP to the 3’-OH ends of double or single stranded DNA. This method is often called TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) or end labeling. Sites where the Br-dUTP is incorporated can then be detected with an antibody specific to BrdU. With the APO-BrdUTM Kit cells are first labeled with Br-dUTP, and then sites of incorporation are detected through staining with a FITC anti-BrdU antibody. Samples can then be analyzed via flow cytometry. Samples that are apoptotic will stain brightly with the anti-BrdU antibody due to the substantial number of exposed 3’-OH sites, while cells that are non-apoptotic will not have incorporated significant amounts of Br-dUTP and will stain dimly. The APO-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.

Katalogové číslo: TNB-6600-KIT

Měrná jednotka: 1 * 50 Tests

Dodavatel: Tonbo Biosciences

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-6584R-A350

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-1721R-CY7

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-6584R-FITC

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-6584R-A488

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-6584R-A647

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-1721R

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-1721R-FITC

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-1721R-CY5

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-6584R-CY7

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-6584R

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-1721R-CY5.5

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce

Popis: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Katalogové číslo: BOSSBS-1721R-CY3

Měrná jednotka: 1 * 100 µl

Dodavatel: Bioss

Akce