Hledali jste: NS-2028

Dodavatel: Cytiva

Popis: The illustra™ plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. Produces high quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.

Katalogové číslo: (GENESIGEASY-EK)

Dodavatel: PRIMERDESIGN

Popis: The genesig easy DNA/RNA extraction protocol begins with a simple lysis step where cells and tissue are lysed to release their nucleic acid. Then minute magnetic particles are added to bind to DNA/RNA. When placed on to the genesig magnetic separator the particles are pulled to the side of the tube making it easy to remove the unwanted supernatant with a pipette. Then a series of simple wash steps are performed before the DNA/RNA is washed off the beads back in to solution, ready for analysis by real-time PCR.

It is fast, and incredibly easy to perform.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Cytiva

Popis: illustra™ tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from a variety of animal tissues and mammalian cell cultures. The kit can be used for several downstream applications including restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR.

Dodavatel: OMEGA BIO-TEK

Popis: The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

Bezpečnostní list Certifikáty

Katalogové číslo: (70748-3)

Dodavatel: Merck Millipore (Novagen)

Popis: Pellet Paint® NF Co-Precipitant is a non-fluorescent dye-labelled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye® Terminators during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualised carrier provides a simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labelled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Re-suspension of pelleted sequencing reaction products in deionised formamide can be confirmed by checking for dissolution of the carrier pellet.

Měrná jednotka: 1 * 125 Tests

Akce

Katalogové číslo: (786-355)

Dodavatel: G-Biosciences

Popis: GET™ Plant DNA Template is a spin column format kit suitable for the preparation of DNA templates from plant samples. The isolated genomic DNA is suitable for PCR and other applications. GET™ Plant DNA Template purifies plant genomic DNA free from plant impurities known to inhibit downstream applications. The high yield, extracted DNA is normally >50 kb in size. The yield is ~30 µg DNA per preparation, depending on the plant source and quantity used.

Měrná jednotka: 1 * 100 Tests

Akce

Katalogové číslo: (116558050.)

Dodavatel: MP Biomedicals

Popis: <p>SPINeasy® DNA Kit for Tissue (with Lysing Matrix) is a high-performance genomic DNA (gDNA) extraction kit which combines mechanical, chemical and enzymatic disruption of tissue samples in 30 minutes. The addition of proteinase K allows a more efficient and complete digestion of any tissue type. Homogenizing time is greatly reduced while substantially increasing the gDNA yield and purity. The kit is validated for tissue samples of fatty, tough, and fibrous samples, and suitable for downstream applications such as PCR and qPCR.</p>

Měrná jednotka: 1 * 50 Tests

New Product Akce

Dodavatel: Sartorius

Popis: LentiSELECT kits offer LentiSELECT 40, LentiSELECT 500 and LentiSELECT 1000 for the purification and concentration of VSV-G pseudotyped lentivirus from 40 to 1000 ml cell culture, leading to purified infective particles. These lentivirus purification and concentration kits offer purified recombinant lentivirus particles for <i>in-vitro</i> transfection a fast, safe and easy to use solution. These kits replace time-consuming ultracentrifugation protocols, which typically take about one day for large sample volumes, thus reducing the purification time to only a few hours.

Dodavatel: Thermo Fisher Scientific

Popis: GeneJET™ Plasmid Maxiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. GeneJET™ Plasmid Maxiprep Kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

Dodavatel: Corning

Popis: AxyPrep™ Mag Plasmid Purification Kit utilises a magnetic bead technology for high throughput purification of plasmid DNA from <i>E.coli</i> cells. The AxyPrep™ Mag kit can also be used with fosmid and BAC vector-based constructs ranging from 5 to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralisation, DNA binding, ethanol washing and plasmid elution. The protocol is automation-compatible.

Certifikáty

Dodavatel: Quantabio

Popis: Extracta™ DBS is a ready to use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman® 903 filter/TFN or 226 from Ahlstrom Munksjö paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix® or PerfeCTa MultiPlex qPCR ToughMix® enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

Bezpečnostní list

Dodavatel: Cytiva

Popis: The illustra™ bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from gram-positive and gram-negative bacteria. The kit can be used to isolate genomic DNA from a wide range of bacterial strains such as DH5a, TOP10, JM109, <i>Bacillus subtilis</i>, etc. The illustra™ bacteria genomicPrep Mini Spin Kit produces highly pure and intact genomic DNA with A₂₆₀/A₂₈₀ values of 1,75 to 1,85.

Katalogové číslo: (69049-3)

Dodavatel: Merck Millipore (Novagen)

Popis: Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.

Měrná jednotka: 1 * 125 Tests

Akce

Katalogové číslo: (AXYGMAGPLANTGDNA10)

Dodavatel: Corning

Popis: Axygen® AxyPrep™ Mag Plant gDNA Kit is a magnetic bead-based purification system that allows rapid and reliable isolation of high quality gDNA from a wide variety of plant samples. Up to 96 samples (50 mg fresh tissue per sample or 15 mg of dried tissue per sample) can be processed in less than one hour. The Axygen® AxyPrep™ Mag Plant gDNA Kit eliminates polysaccharides, phenolic compounds and enzyme inhibitors with no organic extractions. This kit is adaptable for use on major liquid handling workstations for high throughput processing.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (K0761)

Dodavatel: Thermo Fisher Scientific

Popis: GeneJET™ Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids. The kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Thermo Fisher Scientific

Popis: GeneJET™ Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (for example, young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, Southern blotting, and enzymatic reactions.