Hledali jste: 5(6)-FAM+cadaverine

Katalogové číslo: (PRDER01321)

Dodavatel: PRIMERDESIGN

Popis: This genesig® Easy qPCR detection kit targets the invasion protein (invA) gene from <i>Salmonella spp. </i>The kit is designed for the <i>in vitro</i> quantification of<i> Salmonella spp</i> genomes and to have a broad detection profile.

Měrná jednotka: 1 * 50 Tests

New Product Akce

Dodavatel: BIOPREMIER

Popis: Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific pathogenic bacteria. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target microorganism. PCR is a method used to amplify a specific DNA sequence which is typically amplified in a reaction containing a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence. When this solution is heated, the DNA molecule denatures, separating into two strands. As the solution cools, the primers anneal to the target sequences in the separated DNA strands and the DNA polymerase synthesises a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons). When repeated, this cycle of denaturing, annealing, and extending exponentially increases the number of target amplicons. In Real-Time PCR, specific fluorescent probes are used to detect the amplified DNA by hybridising with amplicons. These probes are linked to a fluorophore on one end and a quencher that suppresses fluorescence on the other. If the target sequence is present during the PCR, amplification occurs and the probe is degraded, resulting in fluorescence increase. Fluorescence is measured by a detector and the associated software plots the fluorescence intensity versus number of cycles, allowing the determination of the presence or absence of the target organism.

Certifikáty

Dodavatel: BIOPREMIER

Popis: Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of an animal species in meat and meat products. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target organism. According to European Commission Directive 2002/86/EC, food ingredients have to be declared. In the case of meat and meat products, the animal species has to be disclosed on the label. Moreover, the species autenticity can be highly relevant to consumers for economic, medical, cultural and religious reasons. Fraudulent substitution of cheaper meats in place of more expensive species, the inclusion of meat in non meat (vegetarian) products and the presence of allergens in food products are clear examples for the importance of this issue (Ballin, 2010; Dooley et al., 2004). Several methods are based on identification of proteins by means of electrophoretic and/or immunological methods. However, these methods are not reliable for application in highly processed and heated products due to the protein deterioration. DNA is more stable than proteins during processing and although it can be fragmented by several processes, modern DNA methodologies like PCR-based techniques still allow the identification of DNA from the different species present in a sample (Lockley & Bardsley, 2000). Real-Time PCR techniques are especially suitable for these products because small fragments of DNA can still be amplified and identified with high sensitivity and specificity (Dooley et al., 2004).

Dodavatel: Biotium

Popis: EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.