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Popis: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Katalogové číslo: GENORC-212

Měrná jednotka: 1 * 1 g

Dodavatel: G-Biosciences

Akce

Popis: Adapter, Centri-Lab, 4x50 ml, type C, Centrifuge, Benchtop

Katalogové číslo: 521-1828

Měrná jednotka: 1 * 1 KS

Dodavatel: Thermo Scientific

Akce

Popis: Anti-PTEN Rabbit Polyclonal Antibody

Katalogové číslo: USBI040620

Měrná jednotka: 1 * 200 µl

Dodavatel: US Biological

Akce

Popis: Eskulin seskihydrát

Katalogové číslo: J63114.14

Měrná jednotka: 1 * 25 g

Dodavatel: Thermo Fisher Scientific

Bezpečnostní list Certifikáty Akce

Popis: IPTG (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli.
IPTG is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal in blue-white color selection of recombinant bacterial colonies.
The final concentration typically used in indicator plates is 0.2 mM.

Katalogové číslo: ICNA0210210101

Měrná jednotka: 1 * 1 g

Dodavatel: MP Biomedicals

Akce

Popis: Anti-PTEN Rabbit Polyclonal Antibody

Katalogové číslo: USBI146059

Měrná jednotka: 1 * 100 µG

Dodavatel: US Biological

Akce

Popis: Anti-PTEN Rabbit Polyclonal Antibody (Biotin)

Katalogové číslo: USBI040615-BIOTIN

Měrná jednotka: 1 * 200 µl

Dodavatel: US Biological

Akce

Popis: Anti-PTEN Rabbit Polyclonal Antibody (Biotin)

Katalogové číslo: USBI040611-BIOTIN

Měrná jednotka: 1 * 200 µl

Dodavatel: US Biological

Akce

Popis: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Katalogové číslo: ICNA0219481110

Měrná jednotka: 1 * 10 mg

Dodavatel: MP Biomedicals

Akce

Popis: X-GlcA ((5-bromo-4-chloro-3-indolyl-ß-D-glucuronide) cyclohexylammonium salt) 98%

Katalogové číslo: ACRO331191000

Měrná jednotka: 1 * 100 mg

Dodavatel: Thermo Fisher Scientific

Bezpečnostní list Akce

Popis: X-GlcA ((5-bromo-4-chloro-3-indolyl-ß-D-glucuronide) cyclohexylammonium salt)

Katalogové číslo: A1113.0001

Měrná jednotka: 1 * 1 g

Dodavatel: PanReac AppliChem

Akce

Popis: X-GlcA is a substrate for b-Glucuronidase (GUS) encoded by the gusA gene. The substrate is used as a qualitative marker of specific GUS expression.

Katalogové číslo: APOSBIMB1021-250MG

Měrná jednotka: 1 * 250 mg

Dodavatel: Apollo Scientific

Akce

Popis: X-GlcA ((5-bromo-4-chloro-3-indolyl-ß-D-glucuronide) cyclohexylammonium salt)

Katalogové číslo: 10016.

Měrná jednotka: 1 * 25 mg

Dodavatel: Biotium

Akce

Popis: [EN](2R,3R,4R,5S)-1-BUTYL-2-(HYDROXYMETH 1 * 50 mg

Katalogové číslo: APOSOR9100T-50MG

Měrná jednotka: 1 * 50 mg

Dodavatel: Apollo Scientific

New Product Akce

Popis: [EN]TIP EJECTOR, PEQPETTE 20E + 100E (CL 1 * 1 KS

Katalogové číslo: VWRISP19077

Měrná jednotka: 1 * 1 KS

Dodavatel: VWR Collection

Akce

Popis: TRIMETHOPRIM 4-OL (25 MG) (2-AMINO-5-(3, 1 * 25 mg

Katalogové číslo: 1A05950

Měrná jednotka: 1 * 25 mg

Dodavatel: USP

New Product Akce