Hledali jste: Dimethyl+azelate

Popis: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

Katalogové číslo: L0475-500

Měrná jednotka: 1 * 500 mL

Dodavatel: Biowest

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Popis: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Katalogové číslo: X0920-100

Měrná jednotka: 1 * 100 mL

Dodavatel: Biowest

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Popis: Glutamine penicillin streptamycin acts against gram-negative and gram-positive bacteria.

Katalogové číslo: L0014-100

Měrná jednotka: 1 * 100 mL

Dodavatel: Biowest

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Popis: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Katalogové číslo: P0134-N10L

Měrná jednotka: 1 * 1 KS

Dodavatel: Biowest

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Popis: These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells <i>in vitro</i>. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Katalogové číslo: L0615-C10LS

Měrná jednotka: 1 * 10 L

Dodavatel: Biowest

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Popis: Versene can be used instead of trypsin. It is a chelating agent that disperses the cells by cutting the cytoplasmic bridges between them. Versene has the advantage of being able to withstand autoclave temperature, which is a guarantee of sterility. In addition, it is less aggressive cells than trypsin, which is useful for studies on cell growth.

Katalogové číslo: L0630-100

Měrná jednotka: 1 * 100 mL

Dodavatel: Biowest

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Popis: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

Katalogové číslo: P0191-N50L

Měrná jednotka: 1 * 1 KS

Dodavatel: Biowest

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Popis: Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Katalogové číslo: X0557-100

Měrná jednotka: 1 * 100 mL

Dodavatel: Biowest

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Popis: Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.

Katalogové číslo: L0009-020

Měrná jednotka: 1 * 20 mL

Dodavatel: Biowest

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Popis: Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

Katalogové číslo: L0040-020

Měrná jednotka: 1 * 20 mL

Dodavatel: Biowest

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Popis: L(+)-Glutamin 0.2 mol/l vodný roztok

Katalogové číslo: X0550-100

Měrná jednotka: 1 * 100 mL

Dodavatel: Biowest

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Popis: McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Katalogové číslo: P0390-N1L

Měrná jednotka: 1 * 1 KS

Dodavatel: Biowest

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Popis: Penicillin-Streptomycin acts against gram-negative, gram-positive bacteria and mycoplasma.

Katalogové číslo: L0018-100

Měrná jednotka: 1 * 100 mL

Dodavatel: Biowest

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Popis: Antibiotic-antimycotic used in tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi. Penicillin acts by inhibiting bacterial cell wall synthesis.

Katalogové číslo: L0010-020

Měrná jednotka: 1 * 20 mL

Dodavatel: Biowest

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Popis: Cell culture water is high-quality water suitable for use as a solvent in the preparation of cell culture media and laboratory reagents.

Katalogové číslo: L0970-100

Měrná jednotka: 1 * 100 mL

Dodavatel: Biowest

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Popis: Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, <i>Drosophila melanogaster</i>. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

Katalogové číslo: L0207-500

Měrná jednotka: 1 * 500 mL

Dodavatel: Biowest

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