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3,3',5,5'-Tetramethylbenzidin ≥99%, Sigma-Aldrich®

Dodavatel: Merck

Synonyms: TMB, 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine

M-Clarity™ quality level = MQ200

860336-100MG 860336-1G 860336-25G
SIAL860336-100MGEA 776 CZK
SIAL860336-100MG SIAL860336-1G SIAL860336-25G
3,3',5,5'-Tetramethylbenzidin ≥99%, Sigma-Aldrich®
3,3',5,5'-Tetramethylbenzidin

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures.


The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Vzorec: C₁₆H₂₀N₂
MW: 240,35 g/mol
Bod varu: 402 °C (1013 hPa)
Bod tání: 166…170 °C
Storage Temperature: Ambient
MDL Number: MFCD00007748
CAS číslo: 54827-17-7
EINECS: 259-364-6

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