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VWR®, Fast HiFi DNA polymerase, 2X master mix

Dodavatel: VWR Chemicals

733-2884EA 5590 CZK
733-2884 733-2885 733-2886 733-2887
VWR®, Fast HiFi DNA polymerase, 2X master mix
Činidla pro nukleové kyseliny End-point PCR Enzymes and Kits

Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.


  • All-in-one 2X master mix for great convenience
  • High fidelity: >60X Taq Fidelity
  • 3’→5’ proofreading exonuclease activity
  • Processes up to 11 kb from complex templates (e.g. human gDNA)
  • Good coverage on DNA templates with low to high GC content
  • Fast extension rate: 10 s/kb


Fast HiFi DNA Polymerase 2X master mix is a ready to use 2X reaction mix composed of Fast HiFi DNA Polymerase and an optimised buffer system including dNTPs and magnesium chloride, allowing robust amplification on DNA target with low to high GC content and long DNA targets. Fast HiFi DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proof reading exonuclease activity enabling this polymerase to correct base pair mismatches.


For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 to 2 M Betaine enhancer solution is recommended.


Fast HiFi DNA Polymerase 2X master mix is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.


Sample types: gDNA, plasmid DNA, bacterial DNA, λDNA and other DNA templates

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Produktová specifikace

Popis Fast HiFi DNA Polymerase 2x Master Mix 100 reactions Fast HiFi DNA Polymerase 2x Master Mix 500 reactions Fast HiFi DNA Polymerase 2x Master Mix 2500 reactions Fast HiFi DNA Polymerase 2x Master Mix 5000 reactions
Formát Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs
Environmentally Preferable
Funkce feature-ls-33964141|feature-ls-33964143 feature-ls-33964141|feature-ls-33964143 feature-ls-33964141|feature-ls-33964143 feature-ls-33964141|feature-ls-33964143
Horký start No No No No
Koncentrace 2x 2x 2x 2x
Jednotky 100 r Mix 100 500 r Mix 500 2500 r Mix 2500 5000 r Mix 5000
Rozměry 2 x 1.25 ml 10 x 1.25 ml 50 x 1.25 ml 25 x 5 ml
Vysoká kvalita reprodukce zvuku Ano Ano Ano Ano
Velikost amplikonu 11 kb for gDNA 11 kb for gDNA 11 kb for gDNA 11 kb for gDNA
Processivity Fast Fast Fast Fast
Aktivita exonukleázy 5’→3’ 3’→5’ 5’→3’ 3’→5’ 5’→3’ 3’→5’ 5’→3’ 3’→5’
Product overhang No No No No
Podmínky uskladnění – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months)
Aplikace Cloning (high fidelity),Standard amplification Cloning (high fidelity),Standard amplification Cloning (high fidelity),Standard amplification Cloning (high fidelity),Standard amplification

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