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Betaine enhancer solution 5 M (5X) for PCR

Dodavatel: VWR Chemicals

Synonyms: TMG

733-1361EA 2070 CZK
733-1361 733-2450 733-2451
Betaine enhancer solution 5 M (5X) for PCR
Betaine

Betainový enhancer je účinný zejména při použití s templáty bohatými na CG oblasti nebo s templáty s vysokým stupněm sekundárních struktur. Snižuje teplotu tání primerů (melting temperature).


  • Zlepšuje amplifikaci na GC bohatých a obtížných cílech DNA
  • Snižuje teplotu tání


The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.

Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction.


Upozornení: For Research Use Only. Not for use in diagnostic procedures.

Vzorec: C₅H₁₁NO₂
MW: 117.15 g/mol
Storage Temperature: Freezer
MDL Number: MFCD00012123
CAS číslo: 107-43-7

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Výsledky specifikačního testu

Functional test - 1x Betaine Enchancer Solution is tested for amplification of 613bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions Passes test
Volume check- fill volume >= 0.1ml per tube Passes test


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