Hledali jste: Western Blotting Reagents

Dodavatel: Thermo Fisher Scientific

Popis: Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

Dodavatel: Thermo Fisher Scientific

Popis: The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Katalogové číslo: (PIER34035)

Dodavatel: Thermo Fisher Scientific

Popis: NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.

Měrná jednotka: 1 * 1 g

Akce

Dodavatel: Thermo Fisher Scientific

Popis: Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.

Katalogové číslo: (PIER37574)

Dodavatel: Thermo Fisher Scientific

Popis: The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.

Měrná jednotka: 1 * 250 mL

Akce

Dodavatel: Thermo Fisher Scientific

Popis: LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.

Dodavatel: Thermo Fisher Scientific

Popis: The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

Katalogové číslo: (PIER34012)

Dodavatel: Thermo Fisher Scientific

Popis: Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.

Měrná jednotka: 1 * 250 mL

Akce

Dodavatel: AAT BIOQUEST

Popis: Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

New Product

Katalogové číslo: (PIER15162)

Dodavatel: Thermo Fisher Scientific

Popis: QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (PIER34042)

Dodavatel: Thermo Fisher Scientific

Popis: The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and <i>in situ</i> hybridisation applications.

Měrná jednotka: 1 * 250 mL

Akce

Katalogové číslo: (786-423)

Dodavatel: G-Biosciences

Popis: High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.

Měrná jednotka: 1 * 1 L

Akce

Katalogové číslo: (PIER34070)

Dodavatel: Thermo Fisher Scientific

Popis: The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and <i>in situ</i> hybridisation applications.

Měrná jednotka: 1 * 100 mL

Akce

Katalogové číslo: (PIER34000)

Dodavatel: Thermo Fisher Scientific

Popis: Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (PIER34002)

Dodavatel: Thermo Fisher Scientific

Popis: DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (PIER17075)

Dodavatel: Thermo Fisher Scientific

Popis: North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.

Měrná jednotka: 1 * 1 KIT

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