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Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Product Image-M334-100ML

VWR® Lysis Buffer, Hypotonic

Katalogové číslo: (M334-100ML)
Dodavatel: VWR Chemicals
Popis: Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.
Měrná jednotka: 1 * 100 mL
Product Image-G193-05

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Dodavatel: Avantor
Popis: J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Environmentally Preferable

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Image Unavailable-ENZOBMLKI3460020

HDAC cell lysis buffer

Katalogové číslo: (ENZOBMLKI3460020)
Dodavatel: ENZO LIFE SCIENCES
Popis: Cell lysis buffer for Fluor de Lys HDAC assays
Měrná jednotka: 1 * 20 mL

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Product Image-786-579

Gram Negative lysis buffer

Dodavatel: G-Biosciences
Popis: Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including <i>E.coli</i> strains.

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E.Z.N.A.® RNALock stabilizer reagent

Dodavatel: OMEGA BIO-TEK
Popis: The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

Bezpečnostní list Certifikáty

Image Unavailable-ENZOADI801339

Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Katalogové číslo: (ENZOADI801339)
Dodavatel: ENZO LIFE SCIENCES
Popis: Cell lysis buffer 4, Enzo Life Sciences
Měrná jednotka: 1 * 100 mL

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Image Unavailable-ENZOADI801496

Cell lysis buffer, RIPA

Katalogové číslo: (ENZOADI801496)
Dodavatel: ENZO LIFE SCIENCES
Popis: The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.
Měrná jednotka: 1 * 100 mL

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Product Image-TNB-4300-L100

RBC Lysis Buffer (10X)

Katalogové číslo: (TNB-4300-L100)
Dodavatel: Tonbo Biosciences
Popis: RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.
Měrná jednotka: 1 * 100 mL

Certifikáty


Product Image-X193-01

J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Dodavatel: Avantor
Popis: J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

Certifikáty Environmentally Preferable

Image Unavailable-ENZOADI801526

Extraction reagents

Dodavatel: ENZO LIFE SCIENCES
Popis: Concentrated lysis buffer for preparation of cell and tissue extracts.

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Image Unavailable-ENZOADI801284

RIPA cell lysis buffer 2

Katalogové číslo: (ENZOADI801284)
Dodavatel: ENZO LIFE SCIENCES
Popis: RIPA cell lysis buffer 2, Enzo Life Sciences
Měrná jednotka: 1 * 100 mL

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Product Image-786-263

Research buffers and reagents

Dodavatel: G-Biosciences
Popis: These DNase- and RNase-free buffers and reagents are designed for high quality research.

Image Unavailable-ENZOBMLKI1170030

Caspase cell lysis buffer

Katalogové číslo: (ENZOBMLKI1170030)
Dodavatel: ENZO LIFE SCIENCES
Popis: Caspase cell lysis buffer, Enzo Life Sciences
Měrná jednotka: 1 * 30 mL

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Product Image-78840.

Subcellular Protein Fractionation Kit for Cultured Cells

Katalogové číslo: (78840.)
Dodavatel: Thermo Fisher Scientific
Popis: This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
Měrná jednotka: 1 * 1 KIT
Product Image-786-181T

Protein extraction kit, tissue PE LB™

Dodavatel: G-Biosciences
Popis: Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Product Image-786-180

Protein extraction kit, mammalian cell PE LB™

Katalogové číslo: (786-180)
Dodavatel: G-Biosciences
Popis: Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
Měrná jednotka: 1 * 500 mL
Cena na vyžádání
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Daná chemikálie je regulována dle platné legislativy a bude vyžadováno vyplnění formuláře. Jeho včasným vyplněním uspíšíte dodání produktu.
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
Tento produkt je zablokován vaší organizací. Kontaktujte své nákupní oddělení pro více informací.
Původní produkt již není k dispozici. Zobrazen je náhradní produkt.
Tento produkt již není k dispozici. Podobné produkty můžete vyhledat pomocí VWR katalogových čísel nebo čísel dodavatele uvedených výše. Pokud potřebujete další asistenci, zavolejte na telefonní číslo zákaznického servisu VWR 321 570 321.
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