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Product Image-786-652

RNA purification kit, Tri-Xtract™

Dodavatel: G-Biosciences
Popis: Tri-Xtract™ is designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridisation, <i>in vitro</i> translation, RNase protection assays and poly (A+) selection.

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dNTP mix

Katalogové číslo: (733-1271)
Dodavatel: Quantabio
Popis: The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.
Měrná jednotka: 1 * 1.000 µl
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Ethidiumbromid

Katalogové číslo: (ICNA04802511)
Dodavatel: MP Biomedicals
Popis: Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands. Biochem/physiol Actions: Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
Měrná jednotka: 1 * 10 mL
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Anti-IFNA2 Mouse Monoclonal Antibody [clone: N27]

Katalogové číslo: (PRSI33-720)
Dodavatel: ProSci Inc.
Popis: Recognises a protein of 16-27 kDa, identified as human Interferon alpha 2 (IFNA2). Its epitope maps between amino acids 43-53 of the IFNA2. This mAb is specific for IFNA2 and does not cross-react with IFNA1. The epitopes of clones N27 and N39 mAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFNA2 after viral infections. The IFNA family consists of 24 or more genes or pseudo-genes. The alpha interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
Měrná jednotka: 1 * 100 µG

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ABsolute™ qPCR Low ROX Mixes

Dodavatel: Thermo Fisher Scientific
Popis: ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

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Anti-IFNA2 Mouse Monoclonal Antibody [clone: N39]

Katalogové číslo: (PRSI33-405)
Dodavatel: ProSci Inc.
Popis: Recognises a protein of 16-27 kDa, identified as human interferon-II) (IFN-(II). Its epitope maps between aa112-148 of IFN-II) (total aa172). This mAb is specific for IFN-(II) and does not cross-react with IFN-(I). The site recognized by this mAb is called "site I" and is responsible for the antiviral and anti-proliferative activities of IFN-(II). Epitopes of N27 and N39 mAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFN- family consists of 24 or more genes or pseudo-genes. IFN-II) is one of the two distinct families (I and II) of human IFN-. The -interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
Měrná jednotka: 1 * 100 µG

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VWR®, Accessories for peqGOLD Kits

Dodavatel: VWR Chemicals
Popis: Nucleic Acid Purification Kits and Reagents, Solution I

Bezpečnostní list Certifikáty

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Formamid ≥99.5% (by GC), Ultrapure Bioreagent for denaturing nucleic acids prior to analysis, J.T.Baker®

Dodavatel: Avantor
Popis: For denaturing nucleic acids prior to analysis.

Certifikáty

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Fenol ≥99% contains no preservatives, white fused crystals, Ultrapure Bioreagent for extraction of nucleic acids, J.T.Baker®

Dodavatel: Avantor
Popis: Fused white crystals. For extraction of nucleic acids. Packaged under nitrogen. Lot analysis on label.

Product Image-ENZOENZ450150100

EpiXtract® Nuclear protein isolation kit II (nucleic acid-free)

Katalogové číslo: (ENZOENZ450150100)
Dodavatel: ENZO LIFE SCIENCES
Popis: Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.
Měrná jednotka: 1 * 1 KIT
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MagBinder® Fit24 Nucleic Acid Purification System

Katalogové číslo: (B1-001-24)
Dodavatel: OMEGA BIO-TEK
Popis: Elevate your laboratory experience with the MagBinder® Fit24 Nucleic acid purification system. Efficiency, affordability, precision, and reliability, all in one powerful instrument.
Měrná jednotka: 1 * 1 KS

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Peqlab peqGOLD, DNA Isolation, Bacterial DNA Mini Kit

Dodavatel: VWR Chemicals
Popis: Bacterial DNA Mini Kit for the extraction of high quality DNA from gram-positive and gram-negative bacteria. For up to 1×10⁹ cells per preparation typical yield is up to 30 µg.

Bezpečnostní list Certifikáty

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VWR®, DNA extraction Kit for Real-time PCR food samples analysis

Katalogové číslo: (M04018BF)
Dodavatel: VWR Chemicals
Popis: Rapid method for DNA extraction and purification from all types of microorganisms in a variety of matrices for molecular biology.
Měrná jednotka: 1 * 1 KIT

Certifikáty


Product Image-13-3486-02

Peqlab peqGOLD, DNA Isolation, Plant DNA Mini Kit

Dodavatel: VWR Chemicals
Popis: Dependable isolation of high quality total cellular DNA from a variety of plant species and tissues. peqGOLD homogeniser columns remove cell debris and improve sample handling following lysis.

Bezpečnostní list Certifikáty

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Accessories and consumables for KingFisher™ Flex

Dodavatel: Thermo Fisher Scientific
Popis: Automated nucleic acid purification system, accessory, KingFisher™ Flex hlavice KF na 96 vzorků

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CYGREEN® Nucleic acid Dye

Katalogové číslo: (ENZOENZGEN1050100)
Dodavatel: ENZO LIFE SCIENCES
Popis: Nucleic acid dye for gel staining and higher sensitivity qPCR.
Měrná jednotka: 1 * 100 µl
Cena na vyžádání
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
Daná chemikálie je regulována dle platné legislativy a bude vyžadováno vyplnění formuláře. Jeho včasným vyplněním uspíšíte dodání produktu.
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
Tento produkt je zablokován vaší organizací. Kontaktujte své nákupní oddělení pro více informací.
Původní produkt již není k dispozici. Zobrazen je náhradní produkt.
Tento produkt již není k dispozici. Podobné produkty můžete vyhledat pomocí VWR katalogových čísel nebo čísel dodavatele uvedených výše. Pokud potřebujete další asistenci, zavolejte na telefonní číslo zákaznického servisu VWR 321 570 321.
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