Hledali jste: Materiál pro výuku biologie

Katalogové číslo: (BE-404)

Dodavatel: G-Biosciences

Popis: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-401)

Dodavatel: G-Biosciences

Popis: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (BE-502)

Dodavatel: G-Biosciences

Popis: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO124)

Dodavatel: EDVOTEK

Popis: This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO221)

Dodavatel: EDVOTEK

Popis: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.

Měrná jednotka: 1 * 1 KS

Akce

Dodavatel: EDVOTEK

Popis: Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

Katalogové číslo: (EDVO212)

Dodavatel: EDVOTEK

Popis: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO270)

Dodavatel: EDVOTEK

Popis: This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVOS-49)

Dodavatel: EDVOTEK

Popis: Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitochondrial DNA and their biological father from chromosomal DNA.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO271)

Dodavatel: EDVOTEK

Popis: An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO202)

Dodavatel: EDVOTEK

Popis: Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO282)

Dodavatel: EDVOTEK

Popis: This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO371)

Dodavatel: EDVOTEK

Popis: Students can solve a crime using PCR. Plasmid DNA is provided that, when amplified by PCR, provides products that represent individual DNA profiles.

Měrná jednotka: 1 * 1 KIT

Bezpečnostní list Akce

Katalogové číslo: (EDVOS-48)

Dodavatel: EDVOTEK

Popis: This simulation experiment demonstrates the process of DNA amplification by PCR and how the amplified product is detected by separating the reaction mixture by agarose gel electrophoresis.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EDVO269)

Dodavatel: EDVOTEK

Popis: Students learn the basic principles of the Enzyme-linked Immunosorbent Assay (ELISA) using this precise and sensitive antibody-based detection kit.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (EDVO316)

Dodavatel: EDVOTEK

Popis: Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.

Měrná jednotka: 1 * 1 KIT

Akce