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Dodavatel: Thermo Fisher Scientific

Popis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Dodavatel: OMEGA BIO-TEK

Popis: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

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Dodavatel: Quantabio

Popis: Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

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Dodavatel: Quantabio

Popis: PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.

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Dodavatel: ENZO LIFE SCIENCES

Popis: Optimised standard PCR mix for enhanced speed, yield, and specificity. Used for standard PCR applications.

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Dodavatel: ENZO LIFE SCIENCES

Popis: Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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Katalogové číslo: (ENZOENZ42817)

Dodavatel: ENZO LIFE SCIENCES

Popis: Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for <i>in vitro</i> transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

Měrná jednotka: 1 * 250 Nmo

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Katalogové číslo: (OXNTEXP-RAA114)

Dodavatel: Oxford Nanopore Technologies

Popis: Additional rapid adapter and adapter buffer to enable customers to maximise the use of rapid barcoding kits 24 and 96 V14 (SQK-RBK114.24 and SQK-RBK114.96).

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (40054.)

Dodavatel: Biotium

Popis: Highly purified mix of dNTPs (>99% by HPLC) in 0,6 mM Tris-HCl (pH 7,5); each dNTP is at a concentration of 10 or 25 mm in the solution provided.

Měrná jednotka: 1 * 5 KS

Akce

Katalogové číslo: (GENSE00008)

Dodavatel: Genscript

Popis: Taq DNA polymerase is a thermostable DNA polymerase isolated from an <i>E. coli</i> strain that carries the Taq DNA polymerase gene.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Biotium

Popis: EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

Dodavatel: Quantabio

Popis: An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

Katalogové číslo: (K0512)

Dodavatel: Thermo Fisher Scientific

Popis: DNA purification kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (EP0111)

Dodavatel: Thermo Fisher Scientific

Popis: T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: OMEGA BIO-TEK

Popis: Díky přizpůsobení Hi-Bind® technologie 96-jamkovým destičkám nabízí souprava E-Z 96® Tissue DNA Kit vysoce výkonnou metodu purifikace genomové DNA z krve, bukálních stěrů, myšího ocasu, krysího ocasu, tkání a živočišných buněk a tkání ve formátu 96-jamkové destičky. Purifikovaná DNA je vhodná pro většinu následných aplikací, jako jsou PCR, restrikční enzymatické štěpení a hybridizační techniky.

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Dodavatel: TriLink BioTechnologies

Popis: 2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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