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Hledali jste: Činidla pro extrakci bílkovin

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-786-154

Detergent removal system, DetergentOUT™ GBS10

Dodavatel: G-Biosciences
Popis: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Image Unavailable-786-214

Detergent removal system, DetergentOUT™ Tween®

Dodavatel: G-Biosciences
Popis: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

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Product Image-786-087

Carrier proteins

Dodavatel: G-Biosciences
Popis: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Product Image-PIER90051

Pierce™ EndoProteinase Lys-C Protease, MS Grade

Katalogové číslo: (PIER90051)
Dodavatel: Thermo Fisher Scientific
Popis: Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
Měrná jednotka: 1 * 20 µG
Product Image-786-128

Detergent removal systems, OrgoSol DetergentOUT™

Dodavatel: G-Biosciences
Popis: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Product Image-786-428

Adenine deaminase

Katalogové číslo: (786-428)
Dodavatel: G-Biosciences
Popis: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
Měrná jednotka: 1 * 0,25 mL
Product Image-PIER84841

Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Katalogové číslo: (PIER84841)
Dodavatel: Thermo Fisher Scientific
Popis: The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.
Měrná jednotka: 1 * 10 µG
Product Image-PIER20343

Immobilized pepsin, Pierce™

Katalogové číslo: (PIER20343)
Dodavatel: Thermo Fisher Scientific
Popis: Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
Měrná jednotka: 1 * 5 mL
Product Image-PIER88305

Detergent removal spin column kit, HiPPR™

Dodavatel: Thermo Fisher Scientific
Popis: The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.

Product Image-PIER87780

Pierce™ Detergent Removal Spin Columns and Resin

Dodavatel: Thermo Fisher Scientific
Popis: Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.

Product Image-PIER20341

Immobilised papain, Pierce™

Katalogové číslo: (PIER20341)
Dodavatel: Thermo Fisher Scientific
Popis: Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
Měrná jednotka: 1 * 5 mL
Product Image-786-791

Immobilised Pepsin

Katalogové číslo: (786-791)
Dodavatel: G-Biosciences
Popis: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
Měrná jednotka: 1 * 1 KS
Product Image-786-790

Immobilised Papain

Katalogové číslo: (786-790)
Dodavatel: G-Biosciences
Popis: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
Měrná jednotka: 1 * 1 KS
Product Image-PIER88216

Micrococcal nuclease solution (≥100 U/µl)

Katalogové číslo: (PIER88216)
Dodavatel: Thermo Fisher Scientific
Popis: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
Měrná jednotka: 1 * 1 KS
Product Image-PIER20308

SDS precipitation kit, SDS-Out™

Katalogové číslo: (PIER20308)
Dodavatel: Thermo Fisher Scientific
Popis: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
Měrná jednotka: 1 * 1 KIT
Product Image-ICNA116540438

Lysing matrix Z, MP Biomedicals

Dodavatel: MP Biomedicals
Popis: Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.

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Omezené množství produktu na skladě. Zboží může být k dispozici v jiném skladě poblíž vašeho sídla. Přesvědčte se, že jste přihlášení na stránky, abyste mohli vidět dostupné položky na skladě. Pokud je stále zobrazeno call a potřebujete asistenci, volejte na číslo 321 570 321.
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