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Dodavatel: Biotium

Popis: Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.

Katalogové číslo: (CAYM10006915-96)

Dodavatel: Cayman Chemical

Popis: A 96-well assay for measurement of PPARα DNA binding activity.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Tonbo Biosciences

Popis: Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.

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Dodavatel: Biotium

Popis: MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

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Katalogové číslo: (CAYM600020-96)

Dodavatel: Cayman Chemical

Popis: 96-Well assay for measurement of p53.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (CAYM10006914-96)

Dodavatel: Cayman Chemical

Popis: A 96-well assay for measurement of PPARδ DNA binding activity.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (CAYM10006910-96)

Dodavatel: Cayman Chemical

Popis: HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Biotium

Popis: CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.

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Katalogové číslo: (CAYM601130-1)

Dodavatel: Cayman Chemical

Popis: Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

Měrná jednotka: 1 * 1 KIT

Akce

Katalogové číslo: (CAYM11076-50)

Dodavatel: Cayman Chemical

Popis: For screening a variety of epigenetic compounds.

Měrná jednotka: 1 * 50 µl

Akce

Katalogové číslo: (CAYM10010721-1)

Dodavatel: Cayman Chemical

Popis: A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.

Měrná jednotka: 1 * 1 KS

Akce

Katalogové číslo: (30072.)

Dodavatel: Biotium

Popis: This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

Měrná jednotka: 1 * 1 KIT

Akce

Dodavatel: Biotium

Popis: TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

Katalogové číslo: (API-16S)

Dodavatel: G-Biosciences

Popis: Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

Měrná jednotka: 1 * 1 SET

Akce

Katalogové číslo: (80137.)

Dodavatel: Biotium

Popis: CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.

Měrná jednotka: 1 * 1 mg

Akce

Katalogové číslo: (ENZOBMLUW09700001)

Dodavatel: ENZO LIFE SCIENCES

Popis: This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.

Měrná jednotka: 1 * 10 Tests

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