Popis: Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Katalogové číslo: 786-268

Měrná jednotka: 1 * 100 mL

Dodavatel: G-Biosciences

Akce

Popis: Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.

Katalogové číslo: ENZOBMLPW1075A0001

Měrná jednotka: 1 * 10 Tests

Dodavatel: ENZO LIFE SCIENCES

New Product Akce

Popis: Immobilised Protein A and G are designed for binding the constant domains of immunoglobulin (Ig) molecules. Immobilised Protein A is coupled to 6% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. The binding capacity of Protein A is 18 to 43 mg Human IgG per ml resin.

Katalogové číslo: 786-284

Měrná jednotka: 1 * 5 mL

Dodavatel: G-Biosciences

Bezpečnostní list Akce

Popis: STRATEGY™ is designed to allow researchers to rapidly screen a number of purification techniques in a small scale format to identify the system to isolate their protein of interest. The self-contained kit allows researchers to save time and money as separate buffers, resins and columns are not needed.

Katalogové číslo: 786-184HP

Měrná jednotka: 1 * 6 KS

Dodavatel: G-Biosciences

Akce

Popis: For the rapid purification of soluble, 6X His or GST tagged proteins.

Katalogové číslo: 786-630

Měrná jednotka: 1 * 1 KIT

Dodavatel: G-Biosciences

Akce

Popis: The affinity removal spin cartridge reagent kit is designed to remove the most interfering high-abundant protein (albumin) from human samples (such as plasma, serum, urine or cerebrospinal fluid).

Katalogové číslo: AGLS5188-5254

Měrná jednotka: 1 * 1 kt

Dodavatel: AGILENT

Akce

Popis: TEV protease kit that may be used for cleaving proteins or peptides containing the TEV protease recognition sequence E-N-L-Y-F-Q↓(S/G/A/M/C/H).

Katalogové číslo: 29090-1000U

Měrná jednotka: 1 * 1 KS

Dodavatel: Biotium

New Product Akce

Popis: The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Katalogové číslo: 786-802

Měrná jednotka: 1 * 1 KIT

Dodavatel: G-Biosciences

Akce

Popis: The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

Katalogové číslo: 786-803

Měrná jednotka: 1 * 1 KIT

Dodavatel: G-Biosciences

Akce

Popis: The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

Katalogové číslo: 786-173

Měrná jednotka: 1 * 10 KS

Dodavatel: G-Biosciences

Akce

Popis: The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Katalogové číslo: 786-798

Měrná jednotka: 1 * 1 KIT

Dodavatel: G-Biosciences

Akce

Popis: The Melon Gel IgG purification kit offers a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Katalogové číslo: PIER45212

Měrná jednotka: 1 * 1 KIT

Dodavatel: Thermo Fisher Scientific

Akce

Popis: Pierce™ c-Myc Tag IP/Co-IP Kit provides a simple and fast method to study c-Myc-tagged proteins, with advantages over the traditional IP procedure using protein A/G agarose.

Katalogové číslo: PIER23620

Měrná jednotka: 1 * 1 KIT

Dodavatel: Thermo Fisher Scientific

Akce

Popis: The Pierce™ Classic IP Kit uses protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays.

Katalogové číslo: PIER26146

Měrná jednotka: 1 * 1 KIT

Dodavatel: Thermo Fisher Scientific

Akce

Popis: The Pierce™ Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilise IP antibodies onto premium-quality protein A/G magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

Katalogové číslo: PIER88805

Měrná jednotka: 1 * 1 KIT

Dodavatel: Thermo Fisher Scientific

Akce

Popis: Thermo Scientific™ High Select Top14 Abundant Protein Depletion Resin decreases the abundant protein and antibody components of human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.

Katalogové číslo: PIERA36372

Měrná jednotka: 1 * 50 mL

Dodavatel: Thermo Fisher Scientific

Certifikáty Akce