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Fluoro-Jade B (FJB), powder for identifying Degenerating Neurons

Dodavatel: Biosensis

TR-150-FJB
BSENTR-150-FJBEA 13500 CZK
BSENTR-150-FJB
Fluoro-Jade B (FJB), powder for identifying Degenerating Neurons
Fluoro-Jade

The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. The fluorescent dye Fluoro-Jade® B (FJB), like its more purified brother Fluoro-Jade® C (FJC), is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration.


Fluoro-Jade® B differs from FJC in that it is a slightly less refined chemical formulation and thus it does not quite provide the same level of signal to noise or high resolution as FJC. Nonetheless FJB is still widely used and works very well as a marker of degenerating neurons and even glia (see Damjanac M et al., Brain Res. 2007;1128(1):40-9). FJB operates nearly identically in protocol to that of FJC, and Fluoro-Jade® B is compatible with several other labeling procedures including immunofluorescent and fluorescent Nissl techniques Fluoro-Jade B stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade B exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.


Applications: Following our detailed protocol, Fluoro-Jade B labels degenerating neurons which are visualised with blue light excitation, while DAPI (not included) counter stains cell nuclei, visualised with ultra-violet illumination. The Fluoro-Jade B dye can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.


Visualization: FJB visualization is accomplished using blue light or a 488 nm Laser. Excitation Peak: 495 nm. Emission Peak: 521 nm. Filter system for visualizing: Fluorescein/FITC


The dry material is shipped ambient and stable at room temperature during transport.


The powdered dye can be stored desiccated at room temperature in the dark. Storage in a desiccator is recommended as FJB is hydroscopic. The 0.01% stock solution will remain stable for 3 months when stored in a refrigerator, in the dark. The 0.0001-0.0004% working solution in 0.1% acetic acid should be used within 4 hours of preparation. Diluted FJB dye solutions are not stable and should not be stored. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions.

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